The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin E
Background Diazinon (DZN) is an organophosphate pesticide, and nowadays this pesticide is mostly used in agri- culture. In this study, we analyzed the effects of DZN and vitamin E (Vit E) on apoptosis and the proliferation of germ cells in rat testis. Materials and Methods In this experimental study...
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doaj-ff260ca14c0041c6ba0589b66c2584cf2020-11-25T03:59:15ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782019-07-0113215416010.22074/ijfs.2019.561245541The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin EFatemeh Rahimi Anbarkeh0Mohammad Reza Nikravesh1Mehdi Jalali2Hamid Reza Sadeghnia3Zinat Sargazi4Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranResearch Center for Neuroscience, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, IranBackground Diazinon (DZN) is an organophosphate pesticide, and nowadays this pesticide is mostly used in agri- culture. In this study, we analyzed the effects of DZN and vitamin E (Vit E) on apoptosis and the proliferation of germ cells in rat testis. Materials and Methods In this experimental study, 30 male Wistar rats were divided into five groups (n=6 per group) consisting of control, sham (received olive oil), experimental group i (60 mg/kg DZN), experimental group ii (60 mg/kg DZN and 200 mg/kg Vit E), and experimental group iii (200 mg/kg Vit E). After six weeks, left testis of rats was removed for the detection of proliferative cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase end-labeling (TUNEL). Results Compared with the control group, DZN in the experimental group i decreased the number of PCNA-positive cells and increased the number of TUNEL-positive cells (P < 0.001). Vit E improved detrimental changes by the de- crease in the rate of apoptosis and the increase in the proliferation of testicular germ cells (P < 0.001). Conclusion Vit E can decrease the number of TUNEL-positive cells and increase the number of PCNA-positive cells by the neutralization of the toxicity caused by DZN in the testicular tissue.http://www.ijfs.ir/article_45541_5347d34f01a074439f2ebc313dd0801e.pdfapoptosisdiazinonproliferationtestisvitamin e |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fatemeh Rahimi Anbarkeh Mohammad Reza Nikravesh Mehdi Jalali Hamid Reza Sadeghnia Zinat Sargazi |
spellingShingle |
Fatemeh Rahimi Anbarkeh Mohammad Reza Nikravesh Mehdi Jalali Hamid Reza Sadeghnia Zinat Sargazi The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin E International Journal of Fertility and Sterility apoptosis diazinon proliferation testis vitamin e |
author_facet |
Fatemeh Rahimi Anbarkeh Mohammad Reza Nikravesh Mehdi Jalali Hamid Reza Sadeghnia Zinat Sargazi |
author_sort |
Fatemeh Rahimi Anbarkeh |
title |
The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin E |
title_short |
The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin E |
title_full |
The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin E |
title_fullStr |
The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin E |
title_full_unstemmed |
The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin E |
title_sort |
effect of diazinon on cell proliferation and apoptosis in testicular tissue of rats and the protective effect of vitamin e |
publisher |
Royan Institute (ACECR), Tehran |
series |
International Journal of Fertility and Sterility |
issn |
2008-076X 2008-0778 |
publishDate |
2019-07-01 |
description |
Background Diazinon (DZN) is an organophosphate pesticide, and nowadays this pesticide is mostly used in agri- culture. In this study, we analyzed the effects of DZN and vitamin E (Vit E) on apoptosis and the proliferation of germ cells in rat testis. Materials and Methods In this experimental study, 30 male Wistar rats were divided into five groups (n=6 per group) consisting of control, sham (received olive oil), experimental group i (60 mg/kg DZN), experimental group ii (60 mg/kg DZN and 200 mg/kg Vit E), and experimental group iii (200 mg/kg Vit E). After six weeks, left testis of rats was removed for the detection of proliferative cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase end-labeling (TUNEL). Results Compared with the control group, DZN in the experimental group i decreased the number of PCNA-positive cells and increased the number of TUNEL-positive cells (P < 0.001). Vit E improved detrimental changes by the de- crease in the rate of apoptosis and the increase in the proliferation of testicular germ cells (P < 0.001). Conclusion Vit E can decrease the number of TUNEL-positive cells and increase the number of PCNA-positive cells by the neutralization of the toxicity caused by DZN in the testicular tissue. |
topic |
apoptosis diazinon proliferation testis vitamin e |
url |
http://www.ijfs.ir/article_45541_5347d34f01a074439f2ebc313dd0801e.pdf |
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