Antibiotic susceptibility of enterobacteriaceae species isolated from mastitic milk in Algeria

• Main Authors: R Saidi, D Khelef, R Kaidi
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2014-12-01
• Series: Asian Pacific Journal of Reproduction
• Subjects: Antimicrobial resistance; Cow; Mastitis; Escherichia coli; Algeria
• Online Access: http://www.sciencedirect.com/science/article/pii/S2305050014600452

Objective: To determine: species distribution of 35 Enterobacteriaceae isolates involved in bovine mastitis; and antimicrobial susceptibility pattern of all isolates from geographical areas in Algeria. Methods: Enterobacteriaceae species identification was performed based on conventional biochemical techniques and using the API 20 E test. The antibiotic susceptibility was determined according to the National Committee for Clinical Laboratory Standards guidelines (NCCLS). In total, 35 Enterobacteriaceae species: Escherichia coli (E. coli) (n=5), Klebsiella oxytoca (K. oxytoca) (n=5), Enterobacter cloacae (E. cloacae) (n=4), Klebsiella ornithinolytica (K. ornithinolytica) (n=4), Kluyvera spp (n=4), and Hafnia alvei (H. alvei) (n=3), were the most commonly identified. Enterobacteriaceae isolates were the most resistance to Amoxicillin+Clavulanic Acid (74.28%), Ampicillin (28.57%) and Amoxicillin (28.57%) followed by Tetracycline (20%) and Céfodiazin (14.7%). Conclusions: We conclude that Enterobacteriaceae species from bovine milk presented significantly distinct antimicrobial resistance profiles, evaluated by phenotypic test, which has implications for treatment and management decisions.