Characterization of QTLs and Candidate Genes for Days to Heading in Rice Recombinant Inbred Lines
Understanding the gene mechanisms controlling days to heading (DH) is important in rice breeding for adaption in the target environment. Using a recombinant inbred line population derived from the cross between two <i>japonica</i> rice cultivars, Koshihikari and Baegilmi, we identified t...
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doaj-ff5f7a38071842049f01566953690a642020-11-25T03:20:59ZengMDPI AGGenes2073-44252020-08-011195795710.3390/genes11090957Characterization of QTLs and Candidate Genes for Days to Heading in Rice Recombinant Inbred LinesYoungjun Mo0Jong-Min Jeong1Su-Kyung Ha2Jinhee Kim3Changmin Lee4Gung Pyo Lee5Ji-Ung Jeung6National Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaNational Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaNational Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaNational Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaNational Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaDepartment of Integrative Plant Science, Chung-Ang University, Anseong 17546, KoreaNational Institute of Crop Science, Rural Development Administration, Wanju 55365, KoreaUnderstanding the gene mechanisms controlling days to heading (DH) is important in rice breeding for adaption in the target environment. Using a recombinant inbred line population derived from the cross between two <i>japonica</i> rice cultivars, Koshihikari and Baegilmi, we identified three consistent quantitative trait loci (QTLs) for DH for two years, <i>qDH3</i>, <i>qDH6</i>, and <i>qDH7</i> on chromosomes 3, 6, and 7, respectively. While Baegilmi contributed the allele for early heading at <i>qDH6</i> and <i>qDH7</i> with the additive effect of five days each, Koshihikari contributed the allele for early heading at <i>qDH3</i> with the additive effect of three days. Notably, pyramiding two or more alleles for early heading at these QTLs accelerated heading effectively. Sequencing of <i>Hd16</i>, <i>Hd1</i>, and <i>Ghd7</i>, the previously known heading date genes underlying <i>qDH3</i>, <i>qDH6</i>, and <i>qDH7</i>, respectively, revealed that Baegilmi and Koshihikari carry different alleles at the three genes. Molecular markers were developed to screen the allelic compositions of the three genes among 295 Korean commercial rice cultivars. The results showed that few cultivars carry alleles for early heading at the three genes, highlighting that DH can be further accelerated and fine-tuned in breeding programs by combining the desirable alleles of <i>Hd16</i>, <i>Hd1</i>, and <i>Ghd7</i>.https://www.mdpi.com/2073-4425/11/9/957ricedays to headingQTL<i>Hd1</i><i>Ghd7</i><i>Hd16</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Youngjun Mo Jong-Min Jeong Su-Kyung Ha Jinhee Kim Changmin Lee Gung Pyo Lee Ji-Ung Jeung |
spellingShingle |
Youngjun Mo Jong-Min Jeong Su-Kyung Ha Jinhee Kim Changmin Lee Gung Pyo Lee Ji-Ung Jeung Characterization of QTLs and Candidate Genes for Days to Heading in Rice Recombinant Inbred Lines Genes rice days to heading QTL <i>Hd1</i> <i>Ghd7</i> <i>Hd16</i> |
author_facet |
Youngjun Mo Jong-Min Jeong Su-Kyung Ha Jinhee Kim Changmin Lee Gung Pyo Lee Ji-Ung Jeung |
author_sort |
Youngjun Mo |
title |
Characterization of QTLs and Candidate Genes for Days to Heading in Rice Recombinant Inbred Lines |
title_short |
Characterization of QTLs and Candidate Genes for Days to Heading in Rice Recombinant Inbred Lines |
title_full |
Characterization of QTLs and Candidate Genes for Days to Heading in Rice Recombinant Inbred Lines |
title_fullStr |
Characterization of QTLs and Candidate Genes for Days to Heading in Rice Recombinant Inbred Lines |
title_full_unstemmed |
Characterization of QTLs and Candidate Genes for Days to Heading in Rice Recombinant Inbred Lines |
title_sort |
characterization of qtls and candidate genes for days to heading in rice recombinant inbred lines |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2020-08-01 |
description |
Understanding the gene mechanisms controlling days to heading (DH) is important in rice breeding for adaption in the target environment. Using a recombinant inbred line population derived from the cross between two <i>japonica</i> rice cultivars, Koshihikari and Baegilmi, we identified three consistent quantitative trait loci (QTLs) for DH for two years, <i>qDH3</i>, <i>qDH6</i>, and <i>qDH7</i> on chromosomes 3, 6, and 7, respectively. While Baegilmi contributed the allele for early heading at <i>qDH6</i> and <i>qDH7</i> with the additive effect of five days each, Koshihikari contributed the allele for early heading at <i>qDH3</i> with the additive effect of three days. Notably, pyramiding two or more alleles for early heading at these QTLs accelerated heading effectively. Sequencing of <i>Hd16</i>, <i>Hd1</i>, and <i>Ghd7</i>, the previously known heading date genes underlying <i>qDH3</i>, <i>qDH6</i>, and <i>qDH7</i>, respectively, revealed that Baegilmi and Koshihikari carry different alleles at the three genes. Molecular markers were developed to screen the allelic compositions of the three genes among 295 Korean commercial rice cultivars. The results showed that few cultivars carry alleles for early heading at the three genes, highlighting that DH can be further accelerated and fine-tuned in breeding programs by combining the desirable alleles of <i>Hd16</i>, <i>Hd1</i>, and <i>Ghd7</i>. |
topic |
rice days to heading QTL <i>Hd1</i> <i>Ghd7</i> <i>Hd16</i> |
url |
https://www.mdpi.com/2073-4425/11/9/957 |
work_keys_str_mv |
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