Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture

Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM p...

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Main Authors:	Girdhari Rijal, Jing Wang, Ilhan Yu, David R. Gang, Roland K. Chen, Weimin Li
Format:	Article
Language:	English
Published:	MDPI AG 2018-09-01
Series:	International Journal of Molecular Sciences
Subjects:	porcine breast cancer extracellular matrix hydrogel tissue matrix scaffold scaffold 3D culture
Online Access:	http://www.mdpi.com/1422-0067/19/10/2912

id	doaj-ff72d7ad17cb4dd3a33970c257b48eda
record_format	Article
spelling	doaj-ff72d7ad17cb4dd3a33970c257b48eda2020-11-25T00:55:51ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-09-011910291210.3390/ijms19102912ijms19102912Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue CultureGirdhari Rijal0Jing Wang1Ilhan Yu2David R. Gang3Roland K. Chen4Weimin Li5Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, WA 99202, USATissue Imaging and Proteomics Laboratory, Washington State University, Pullman, WA 99164, USASchool of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USATissue Imaging and Proteomics Laboratory, Washington State University, Pullman, WA 99164, USASchool of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USADepartment of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, WA 99202, USAPorcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer.http://www.mdpi.com/1422-0067/19/10/2912porcinebreast cancerextracellular matrixhydrogeltissue matrix scaffoldscaffold3D culture
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Girdhari Rijal Jing Wang Ilhan Yu David R. Gang Roland K. Chen Weimin Li
spellingShingle	Girdhari Rijal Jing Wang Ilhan Yu David R. Gang Roland K. Chen Weimin Li Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture International Journal of Molecular Sciences porcine breast cancer extracellular matrix hydrogel tissue matrix scaffold scaffold 3D culture
author_facet	Girdhari Rijal Jing Wang Ilhan Yu David R. Gang Roland K. Chen Weimin Li
author_sort	Girdhari Rijal
title	Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture
title_short	Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture
title_full	Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture
title_fullStr	Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture
title_full_unstemmed	Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture
title_sort	porcine breast extracellular matrix hydrogel for spatial tissue culture
publisher	MDPI AG
series	International Journal of Molecular Sciences
issn	1422-0067
publishDate	2018-09-01
description	Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer.
topic	porcine breast cancer extracellular matrix hydrogel tissue matrix scaffold scaffold 3D culture
url	http://www.mdpi.com/1422-0067/19/10/2912
work_keys_str_mv	AT girdharirijal porcinebreastextracellularmatrixhydrogelforspatialtissueculture AT jingwang porcinebreastextracellularmatrixhydrogelforspatialtissueculture AT ilhanyu porcinebreastextracellularmatrixhydrogelforspatialtissueculture AT davidrgang porcinebreastextracellularmatrixhydrogelforspatialtissueculture AT rolandkchen porcinebreastextracellularmatrixhydrogelforspatialtissueculture AT weiminli porcinebreastextracellularmatrixhydrogelforspatialtissueculture
_version_	1725229282470395904

Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture

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