Butyrolactone-I from Coral-Derived Fungus Aspergillus terreus Attenuates Neuro-Inflammatory Response via Suppression of NF-κB Pathway in BV-2 Cells
Butyrolactone-I (ZB5-1) from the coral-derived fungus Aspergillus terreus was investigated in this study to estimate its anti-neuroinflammatory effects on lipopolysaccharide (LPS)-induced BV-2 microglia cells. MTT assay indicated that ZB5-1 in tested concentrations had no cytotoxicity on BV-2 cells,...
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doaj-ff79991d5e3b4feca120451fbc295aa62020-11-24T23:08:39ZengMDPI AGMarine Drugs1660-33972018-06-0116620210.3390/md16060202md16060202Butyrolactone-I from Coral-Derived Fungus Aspergillus terreus Attenuates Neuro-Inflammatory Response via Suppression of NF-κB Pathway in BV-2 CellsYuan Yuan Zhang0Yi Zhang1Yuan-Bei Yao2Xiao-Ling Lei3Zhong-Ji Qian4College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaShenzhen Institute of Guangdong Ocean University, Shenzhen 518108, ChinaButyrolactone-I (ZB5-1) from the coral-derived fungus Aspergillus terreus was investigated in this study to estimate its anti-neuroinflammatory effects on lipopolysaccharide (LPS)-induced BV-2 microglia cells. MTT assay indicated that ZB5-1 in tested concentrations had no cytotoxicity on BV-2 cells, and significantly reduced the production of nitric oxide (NO), measured using Griess reagent, and interleukin-1 beta (IL-1β), detected by enzyme-linked immunosorbent assay (ELISA). ZB5-1 also down-regulated the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner by Western blot analysis. Moreover, the effect of ZB5-1 on the nuclear factor-κB (NF-κB) signaling pathway was studied via the expression of phosphorylation of NF-κB p65 and inhibitor of NF-κB (IκB), and the nuclear translocation of NF-κB p65 respectively. The results showed that ZB5-1 could inhibit the phosphorylation of p65 and IκB. Furthermore, molecular docking study suggested that ZB5-1 bound at the active sites of NF-κB to prevent its translocation to the nucleus. Therefore, we suggest ZB5-1 has a potential to reduce the anti-inflammatory response in LPS-induced BV-2 cells.http://www.mdpi.com/1660-3397/16/6/202butyrolactone-IAspergillus terreusmicroglianeuro-inflammation |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuan Yuan Zhang Yi Zhang Yuan-Bei Yao Xiao-Ling Lei Zhong-Ji Qian |
spellingShingle |
Yuan Yuan Zhang Yi Zhang Yuan-Bei Yao Xiao-Ling Lei Zhong-Ji Qian Butyrolactone-I from Coral-Derived Fungus Aspergillus terreus Attenuates Neuro-Inflammatory Response via Suppression of NF-κB Pathway in BV-2 Cells Marine Drugs butyrolactone-I Aspergillus terreus microglia neuro-inflammation |
author_facet |
Yuan Yuan Zhang Yi Zhang Yuan-Bei Yao Xiao-Ling Lei Zhong-Ji Qian |
author_sort |
Yuan Yuan Zhang |
title |
Butyrolactone-I from Coral-Derived Fungus Aspergillus terreus Attenuates Neuro-Inflammatory Response via Suppression of NF-κB Pathway in BV-2 Cells |
title_short |
Butyrolactone-I from Coral-Derived Fungus Aspergillus terreus Attenuates Neuro-Inflammatory Response via Suppression of NF-κB Pathway in BV-2 Cells |
title_full |
Butyrolactone-I from Coral-Derived Fungus Aspergillus terreus Attenuates Neuro-Inflammatory Response via Suppression of NF-κB Pathway in BV-2 Cells |
title_fullStr |
Butyrolactone-I from Coral-Derived Fungus Aspergillus terreus Attenuates Neuro-Inflammatory Response via Suppression of NF-κB Pathway in BV-2 Cells |
title_full_unstemmed |
Butyrolactone-I from Coral-Derived Fungus Aspergillus terreus Attenuates Neuro-Inflammatory Response via Suppression of NF-κB Pathway in BV-2 Cells |
title_sort |
butyrolactone-i from coral-derived fungus aspergillus terreus attenuates neuro-inflammatory response via suppression of nf-κb pathway in bv-2 cells |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2018-06-01 |
description |
Butyrolactone-I (ZB5-1) from the coral-derived fungus Aspergillus terreus was investigated in this study to estimate its anti-neuroinflammatory effects on lipopolysaccharide (LPS)-induced BV-2 microglia cells. MTT assay indicated that ZB5-1 in tested concentrations had no cytotoxicity on BV-2 cells, and significantly reduced the production of nitric oxide (NO), measured using Griess reagent, and interleukin-1 beta (IL-1β), detected by enzyme-linked immunosorbent assay (ELISA). ZB5-1 also down-regulated the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner by Western blot analysis. Moreover, the effect of ZB5-1 on the nuclear factor-κB (NF-κB) signaling pathway was studied via the expression of phosphorylation of NF-κB p65 and inhibitor of NF-κB (IκB), and the nuclear translocation of NF-κB p65 respectively. The results showed that ZB5-1 could inhibit the phosphorylation of p65 and IκB. Furthermore, molecular docking study suggested that ZB5-1 bound at the active sites of NF-κB to prevent its translocation to the nucleus. Therefore, we suggest ZB5-1 has a potential to reduce the anti-inflammatory response in LPS-induced BV-2 cells. |
topic |
butyrolactone-I Aspergillus terreus microglia neuro-inflammation |
url |
http://www.mdpi.com/1660-3397/16/6/202 |
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