Microplasma-assisted hydrogel fabrication: A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application
Toxicity issues and biocompatibility concerns with traditional classical chemical cross-linking processes prevent them from being universal approaches for hydrogel fabrication for tissue engineering. Physical cross-linking methods are non-toxic and widely used to obtain cross-linked polymers in a tu...
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doaj-ff7e43c7b44c41a99ebb99a3dc7826562020-11-25T00:14:45ZengPeerJ Inc.PeerJ2167-83592017-06-015e349810.7717/peerj.3498Microplasma-assisted hydrogel fabrication: A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical applicationMantosh Kumar Satapathy0Wei-Hung Chiang1Er-Yuan Chuang2Chih-Hwa Chen3Jia-Liang Liao4Huin-Ning Huang5Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, TaiwanDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, TaiwanGraduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, TaiwanBone and Joint Research Center, Department of Orthopedics, Taipei Medical University Hospital, School of Medicine, College of Medicine, Taipei, TaiwanDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, TaiwanDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, TaiwanToxicity issues and biocompatibility concerns with traditional classical chemical cross-linking processes prevent them from being universal approaches for hydrogel fabrication for tissue engineering. Physical cross-linking methods are non-toxic and widely used to obtain cross-linked polymers in a tunable manner. Therefore, in the current study, argon micro-plasma was introduced as a neutral energy source for cross-linking in fabrication of the desired gelatin-graphene oxide (gel-GO) nanocomposite hydrogel scaffolds. Argon microplasma was used to treat purified gelatin (8% w/v) containing 0.1∼1 wt% of high-functionality nano-graphene oxide (GO). Optimized plasma conditions (2,500 V and 8.7 mA) for 15 min with a gas flow rate of 100 standard cm3/min was found to be most suitable for producing the gel-GO nanocomposite hydrogels. The developed hydrogel was characterized by the degree of cross-linking, FTIR spectroscopy, SEM, confocal microscopy, swelling behavior, contact angle measurement, and rheology. The cell viability was examined by an MTT assay and a live/dead assay. The pore size of the hydrogel was found to be 287 ± 27 µm with a contact angle of 78° ± 3.7°. Rheological data revealed improved storage as well as a loss modulus of up to 50% with tunable viscoelasticity, gel strength, and mechanical properties at 37 °C temperature in the microplasma-treated groups. The swelling behavior demonstrated a better water-holding capacity of the gel-GO hydrogels for cell growth and proliferation. Results of the MTT assay, microscopy, and live/dead assay exhibited better cell viability at 1% (w/w) of high-functionality GO in gelatin. The highlight of the present study is the first successful attempt of microplasma-assisted gelatin-GO nano composite hydrogel fabrication that offers great promise and optimism for further biomedical tissue engineering applications.https://peerj.com/articles/3498.pdfArgon microplasmaGelatinGraphene oxideCross-linkingHydrogelBiocompatibility |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mantosh Kumar Satapathy Wei-Hung Chiang Er-Yuan Chuang Chih-Hwa Chen Jia-Liang Liao Huin-Ning Huang |
spellingShingle |
Mantosh Kumar Satapathy Wei-Hung Chiang Er-Yuan Chuang Chih-Hwa Chen Jia-Liang Liao Huin-Ning Huang Microplasma-assisted hydrogel fabrication: A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application PeerJ Argon microplasma Gelatin Graphene oxide Cross-linking Hydrogel Biocompatibility |
author_facet |
Mantosh Kumar Satapathy Wei-Hung Chiang Er-Yuan Chuang Chih-Hwa Chen Jia-Liang Liao Huin-Ning Huang |
author_sort |
Mantosh Kumar Satapathy |
title |
Microplasma-assisted hydrogel fabrication: A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application |
title_short |
Microplasma-assisted hydrogel fabrication: A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application |
title_full |
Microplasma-assisted hydrogel fabrication: A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application |
title_fullStr |
Microplasma-assisted hydrogel fabrication: A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application |
title_full_unstemmed |
Microplasma-assisted hydrogel fabrication: A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application |
title_sort |
microplasma-assisted hydrogel fabrication: a novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2017-06-01 |
description |
Toxicity issues and biocompatibility concerns with traditional classical chemical cross-linking processes prevent them from being universal approaches for hydrogel fabrication for tissue engineering. Physical cross-linking methods are non-toxic and widely used to obtain cross-linked polymers in a tunable manner. Therefore, in the current study, argon micro-plasma was introduced as a neutral energy source for cross-linking in fabrication of the desired gelatin-graphene oxide (gel-GO) nanocomposite hydrogel scaffolds. Argon microplasma was used to treat purified gelatin (8% w/v) containing 0.1∼1 wt% of high-functionality nano-graphene oxide (GO). Optimized plasma conditions (2,500 V and 8.7 mA) for 15 min with a gas flow rate of 100 standard cm3/min was found to be most suitable for producing the gel-GO nanocomposite hydrogels. The developed hydrogel was characterized by the degree of cross-linking, FTIR spectroscopy, SEM, confocal microscopy, swelling behavior, contact angle measurement, and rheology. The cell viability was examined by an MTT assay and a live/dead assay. The pore size of the hydrogel was found to be 287 ± 27 µm with a contact angle of 78° ± 3.7°. Rheological data revealed improved storage as well as a loss modulus of up to 50% with tunable viscoelasticity, gel strength, and mechanical properties at 37 °C temperature in the microplasma-treated groups. The swelling behavior demonstrated a better water-holding capacity of the gel-GO hydrogels for cell growth and proliferation. Results of the MTT assay, microscopy, and live/dead assay exhibited better cell viability at 1% (w/w) of high-functionality GO in gelatin. The highlight of the present study is the first successful attempt of microplasma-assisted gelatin-GO nano composite hydrogel fabrication that offers great promise and optimism for further biomedical tissue engineering applications. |
topic |
Argon microplasma Gelatin Graphene oxide Cross-linking Hydrogel Biocompatibility |
url |
https://peerj.com/articles/3498.pdf |
work_keys_str_mv |
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