Study on the Diagnosis of Gastric Cancer by Magnetic Beads Extraction and Mass Spectrometry
Objective. This study constructed a model for the early diagnosis of gastric cancer by comparing the serum peptides profiles of patients with advanced gastric cancer and healthy people. And that model may be the potential to be applied for the efficacy evaluation and recurrence monitoring in gastric...
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doaj-ff8cdfdf272b407ca74124f18d2afa812020-11-25T03:26:37ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/27430602743060Study on the Diagnosis of Gastric Cancer by Magnetic Beads Extraction and Mass SpectrometryNing Zhu0Xiaoliang Xing1Limei Cao2Yingjun Zhang3Ti Zhang4Zhen Li5Fen Zou6Qing Li7Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, School of Public Health and Laboratory Medicine, Hunan University of Medicine, Huaihua, 418000 Hunan, ChinaHunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, School of Public Health and Laboratory Medicine, Hunan University of Medicine, Huaihua, 418000 Hunan, ChinaChenzhou No.1 People’s Hospital, Chenzhou, 423000 Hunan, ChinaHunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, School of Public Health and Laboratory Medicine, Hunan University of Medicine, Huaihua, 418000 Hunan, ChinaHunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, School of Public Health and Laboratory Medicine, Hunan University of Medicine, Huaihua, 418000 Hunan, ChinaHunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, School of Public Health and Laboratory Medicine, Hunan University of Medicine, Huaihua, 418000 Hunan, ChinaSouth China University of Technology, Guangzhou, 510000 Guangdong, ChinaHunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, School of Public Health and Laboratory Medicine, Hunan University of Medicine, Huaihua, 418000 Hunan, ChinaObjective. This study constructed a model for the early diagnosis of gastric cancer by comparing the serum peptides profiles of patients with advanced gastric cancer and healthy people. And that model may be the potential to be applied for the efficacy evaluation and recurrence monitoring in gastric cancer. Methods. Serums of 30 healthy people and 30 advanced gastric cancer patients were matched by age and gender were collected. The serum peptide spectrum was obtained by MB-WCX concentration and MALDI-TOF MS analysis. Based on the analysis of the efficiency of differential peptides in the diagnosis of gastric cancer, we first established a model for the diagnosis of gastric cancer based on differential peptides and then carried out external verification. The diagnostic reliability of this model was further tested by compared with carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9). Results. In this present study, we found the expression of two peptide peaks with a molecular weight of 2863 Da and 2953 Da were significantly increased in gastric cancer serum, while the expression of two peptide peaks with a molecular weight of 1945 Da and 2082 Da were significantly decreased. Depending on the characteristics of peptide expression, we constructed a diagnostic model, we compared the sensitivity and specificity of the model established by 2953 Da/1945 Da, and found this model is significantly higher than CEA and CA19-9. Conclusion. There were some differences in serum peptides profiles between patients with advanced gastric cancer and healthy people. The serum peptide diagnostic models based on 2953 Da and 1945 Da have high diagnostic efficiency for advanced gastric cancer. Our result indicated that this model was well worth further validation for clinical diagnosis.http://dx.doi.org/10.1155/2020/2743060 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ning Zhu Xiaoliang Xing Limei Cao Yingjun Zhang Ti Zhang Zhen Li Fen Zou Qing Li |
spellingShingle |
Ning Zhu Xiaoliang Xing Limei Cao Yingjun Zhang Ti Zhang Zhen Li Fen Zou Qing Li Study on the Diagnosis of Gastric Cancer by Magnetic Beads Extraction and Mass Spectrometry BioMed Research International |
author_facet |
Ning Zhu Xiaoliang Xing Limei Cao Yingjun Zhang Ti Zhang Zhen Li Fen Zou Qing Li |
author_sort |
Ning Zhu |
title |
Study on the Diagnosis of Gastric Cancer by Magnetic Beads Extraction and Mass Spectrometry |
title_short |
Study on the Diagnosis of Gastric Cancer by Magnetic Beads Extraction and Mass Spectrometry |
title_full |
Study on the Diagnosis of Gastric Cancer by Magnetic Beads Extraction and Mass Spectrometry |
title_fullStr |
Study on the Diagnosis of Gastric Cancer by Magnetic Beads Extraction and Mass Spectrometry |
title_full_unstemmed |
Study on the Diagnosis of Gastric Cancer by Magnetic Beads Extraction and Mass Spectrometry |
title_sort |
study on the diagnosis of gastric cancer by magnetic beads extraction and mass spectrometry |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2020-01-01 |
description |
Objective. This study constructed a model for the early diagnosis of gastric cancer by comparing the serum peptides profiles of patients with advanced gastric cancer and healthy people. And that model may be the potential to be applied for the efficacy evaluation and recurrence monitoring in gastric cancer. Methods. Serums of 30 healthy people and 30 advanced gastric cancer patients were matched by age and gender were collected. The serum peptide spectrum was obtained by MB-WCX concentration and MALDI-TOF MS analysis. Based on the analysis of the efficiency of differential peptides in the diagnosis of gastric cancer, we first established a model for the diagnosis of gastric cancer based on differential peptides and then carried out external verification. The diagnostic reliability of this model was further tested by compared with carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9). Results. In this present study, we found the expression of two peptide peaks with a molecular weight of 2863 Da and 2953 Da were significantly increased in gastric cancer serum, while the expression of two peptide peaks with a molecular weight of 1945 Da and 2082 Da were significantly decreased. Depending on the characteristics of peptide expression, we constructed a diagnostic model, we compared the sensitivity and specificity of the model established by 2953 Da/1945 Da, and found this model is significantly higher than CEA and CA19-9. Conclusion. There were some differences in serum peptides profiles between patients with advanced gastric cancer and healthy people. The serum peptide diagnostic models based on 2953 Da and 1945 Da have high diagnostic efficiency for advanced gastric cancer. Our result indicated that this model was well worth further validation for clinical diagnosis. |
url |
http://dx.doi.org/10.1155/2020/2743060 |
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