THE CONDITION OF C-FOS GENE IN THE NEUROSECRETORY NUCLEI OF THE HYPOTHALAMUS IN RATS STRESSED BY LIGHT AND THE EFFECTS OF MELATONIN AND EPITHALON

• Main Authors: R.Ye. Bulyk, A.I. Burachyk, K.V. Vlasova, V.R. Yosypenko
• Format: Article
• Language: English
• Published: Shevchenko Scientific Society 2017-06-01
• Series: Праці Наукового товариства імені Шевченка. Медичні науки
• Subjects: melatonin, epthalon, gene – protein c-fos, epiphysial hypofunction
• Online Access: https://mspsss.org.ua/index.php/journal/article/view/40

The aim was to study the effect of melatonin and a synthetic bioregulator – epthalon for the purpose of correction stress-induced changes of the activity of the gene of “ultraearly response” c-fos in the lateral large cell subnuclei of the paraventricular nucleus (llPVN) of the rat hypothalamus at different intervals of 24- hour period (in the daytime and at night). The expression of the product of this gene – protein c-Fos – in animals kept under normal conditions of alternating illumination and darkness demonstrated a clear-cut circadian pattern (with a higher level by day). The diurnal index of the c-Fos content in the animal’s llPVN is lower by 33,0%, under conditions of light stress, whereas the nocturnal one approximated to the control values. An injection of melatonin (0,5 mg/kg) to light-stressed animals refl ected at 02.00 p.m. hundred by exceeding the index of the c-Fos protein in the animal’s llPVN almost twofold compared to the experimental fi ndings on stressed animals without hormone introduction, as well as by a normalization of the circadian dynamics of the expression of the gene under study. An augmentation of the index of the c-Fos protein concentration was disclosed in the structure upon using tetrapeptide epithalon (0,5 μg/kg) at night in relation to individuals with epiphysial hypofunction without undergoing experimental therapy with epithalon. No such effect was fi xed at night.