Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH
Search for naturally grown food has stimulated the biotechnological production of carotenoids. Therefore, the use of the yeast Xanthophyllomonas dendrorhous has been researched due to its abilities to assimilate different sources as substrates and to produce high amounts of carotenoids. Furthermore,...
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doaj-ff9a98e9e1104634b2db381d3accee972020-11-25T03:03:35ZengUniversity of ZagrebFood Technology and Biotechnology1330-98621334-26062016-01-01544489496Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pHCarolina Moroni Silva0Thais de Matos de Borba1Susana Juliano Kalil2Janaína Fernandes de Medeiros Burkert3Federal University of Rio Grande (FURG), Chemistry and Food School (EQA), PO Box 474, BR-96203-900 Rio Grande, RS, BrazilFederal University of Rio Grande (FURG), Chemistry and Food School (EQA), PO Box 474, BR-96203-900 Rio Grande, RS, BrazilFederal University of Rio Grande (FURG), Chemistry and Food School (EQA), PO Box 474, BR-96203-900 Rio Grande, RS, BrazilFederal University of Rio Grande (FURG), Chemistry and Food School (EQA), PO Box 474, BR-96203-900 Rio Grande, RS, BrazilSearch for naturally grown food has stimulated the biotechnological production of carotenoids. Therefore, the use of the yeast Xanthophyllomonas dendrorhous has been researched due to its abilities to assimilate different sources as substrates and to produce high amounts of carotenoids. Furthermore, alternative sources have been used as the culture medium to reduce costs and environmental impact. A potent carotenoid is astaxanthin in view of its health-promoting and antioxidative properties. It consists of different geometrical isomers with trans and cis configuration. In X. dendrorhous this carotenoid is mostly found in the trans form, but cis isomers can also be found. Carotenoid production was investigated in culture medium containing by-products such as raw glycerol (from biodiesel) and parboiled rice effluent. The effects of the culture medium components on biomass concentration and specific and volumetric productions of carotenoids were verified by the Plackett-Burman design. Cultivations were carried out with yeast Xanthophyllomonas dendrorhous NRRL Y-17268 at 25 °C and 150 rpm for 168 h. In this study, maximum production of carotenoids was obtained under the following conditions (in g/L): raw glycerol 10, glucose 10, yeast extract 10, malt extract 10 and peptone 1 at pH=6. Resulting specific and volumetric productions of carotenoids were 326.8 and 4.1 μg/g, respectively.http://hrcak.srce.hr/file/250796natural pigmentsby-productsPlackett-Burman designXanthophyllomonas dendrorhous |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carolina Moroni Silva Thais de Matos de Borba Susana Juliano Kalil Janaína Fernandes de Medeiros Burkert |
spellingShingle |
Carolina Moroni Silva Thais de Matos de Borba Susana Juliano Kalil Janaína Fernandes de Medeiros Burkert Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH Food Technology and Biotechnology natural pigments by-products Plackett-Burman design Xanthophyllomonas dendrorhous |
author_facet |
Carolina Moroni Silva Thais de Matos de Borba Susana Juliano Kalil Janaína Fernandes de Medeiros Burkert |
author_sort |
Carolina Moroni Silva |
title |
Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH |
title_short |
Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH |
title_full |
Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH |
title_fullStr |
Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH |
title_full_unstemmed |
Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH |
title_sort |
raw glycerol and parboiled rice effluent for carotenoid production: effect of the composition of culture medium and initial ph |
publisher |
University of Zagreb |
series |
Food Technology and Biotechnology |
issn |
1330-9862 1334-2606 |
publishDate |
2016-01-01 |
description |
Search for naturally grown food has stimulated the biotechnological production of carotenoids. Therefore, the use of the yeast Xanthophyllomonas dendrorhous has been researched due to its abilities to assimilate different sources as substrates and to produce high amounts of carotenoids. Furthermore, alternative sources have been used as the culture medium to reduce costs and environmental impact. A potent carotenoid is astaxanthin in view of its health-promoting and antioxidative properties. It consists of different geometrical isomers with trans and cis configuration. In X. dendrorhous this carotenoid is mostly found in the trans form, but cis isomers can also be found. Carotenoid production was investigated in culture medium containing by-products such as raw glycerol (from biodiesel) and parboiled rice effluent. The effects of the culture medium components on biomass concentration and specific and volumetric productions of carotenoids were verified by the Plackett-Burman design. Cultivations were carried out with yeast Xanthophyllomonas dendrorhous NRRL Y-17268 at 25 °C and 150 rpm for 168 h. In this study, maximum production of carotenoids was obtained under the following conditions (in g/L): raw glycerol 10, glucose 10, yeast extract 10, malt extract 10 and peptone 1 at pH=6. Resulting specific and volumetric productions of carotenoids were 326.8 and 4.1 μg/g, respectively. |
topic |
natural pigments by-products Plackett-Burman design Xanthophyllomonas dendrorhous |
url |
http://hrcak.srce.hr/file/250796 |
work_keys_str_mv |
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