Structural elucidation, chain conformation and immuno-modulatory activity of glucogalactomannan from cultured Cordyceps sinensis fungus UM01
The chemical structure, chain conformation, and immuno-modulatory activity of polysaccharide from mycelium Cordyceps sinensis fungus UM01 were investigated. The molecular weights and the intrinsic viscosity of purified polysaccharide (UM01-S4) were determined as 22,559 Da and 5.09 mL/g, respectively...
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doaj-ffaaf6d779a1442d81c7b5509ed5e76a2021-04-30T07:08:02ZengElsevierJournal of Functional Foods1756-46462016-08-0125174185Structural elucidation, chain conformation and immuno-modulatory activity of glucogalactomannan from cultured Cordyceps sinensis fungus UM01Kit-Leong Cheong0Lan-Zhen Meng1Xian-Qiang Chen2Lan-Ying Wang3Ding-Tao Wu4Jing Zhao5Shao-Ping Li6State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, ChinaCorresponding author. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China. Tel.: +853 8822 4873; fax: +853 2884 1358.; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, ChinaThe chemical structure, chain conformation, and immuno-modulatory activity of polysaccharide from mycelium Cordyceps sinensis fungus UM01 were investigated. The molecular weights and the intrinsic viscosity of purified polysaccharide (UM01-S4) were determined as 22,559 Da and 5.09 mL/g, respectively. Using the polymer solution theory, UM01-S4 has a sphere-like conformation in aqueous NaCl at 35 °C. The chemical structure of UM01-S4 contains an α-(1→2)-Manp core. The side chains were composed of β-(1→2)-Galf, β-(1→4)-Glcp, α-GalpA, and α-Manp units, which attached to the mannan core at the O-6 position. The immuno-modulatory assays showed that UM01-S4 exhibited good activities, including macrophages proliferation, phagocytosis, and release of NO and cytokines. The mechanism underlying the macrophage regulation of UM01-S4 might be related to the activation of the MAPK and NF-κB signalling pathways. Thus, mycelium of C. sinensis UM01 could be used as a natural immune-stimulating agent in functional food areas and pharmaceutical industries.http://www.sciencedirect.com/science/article/pii/S1756464616301426Cordyceps sinensisPolysaccharideChain conformationChemical structuralImmuno-modulatory activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kit-Leong Cheong Lan-Zhen Meng Xian-Qiang Chen Lan-Ying Wang Ding-Tao Wu Jing Zhao Shao-Ping Li |
spellingShingle |
Kit-Leong Cheong Lan-Zhen Meng Xian-Qiang Chen Lan-Ying Wang Ding-Tao Wu Jing Zhao Shao-Ping Li Structural elucidation, chain conformation and immuno-modulatory activity of glucogalactomannan from cultured Cordyceps sinensis fungus UM01 Journal of Functional Foods Cordyceps sinensis Polysaccharide Chain conformation Chemical structural Immuno-modulatory activity |
author_facet |
Kit-Leong Cheong Lan-Zhen Meng Xian-Qiang Chen Lan-Ying Wang Ding-Tao Wu Jing Zhao Shao-Ping Li |
author_sort |
Kit-Leong Cheong |
title |
Structural elucidation, chain conformation and immuno-modulatory activity of glucogalactomannan from cultured Cordyceps sinensis fungus UM01 |
title_short |
Structural elucidation, chain conformation and immuno-modulatory activity of glucogalactomannan from cultured Cordyceps sinensis fungus UM01 |
title_full |
Structural elucidation, chain conformation and immuno-modulatory activity of glucogalactomannan from cultured Cordyceps sinensis fungus UM01 |
title_fullStr |
Structural elucidation, chain conformation and immuno-modulatory activity of glucogalactomannan from cultured Cordyceps sinensis fungus UM01 |
title_full_unstemmed |
Structural elucidation, chain conformation and immuno-modulatory activity of glucogalactomannan from cultured Cordyceps sinensis fungus UM01 |
title_sort |
structural elucidation, chain conformation and immuno-modulatory activity of glucogalactomannan from cultured cordyceps sinensis fungus um01 |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2016-08-01 |
description |
The chemical structure, chain conformation, and immuno-modulatory activity of polysaccharide from mycelium Cordyceps sinensis fungus UM01 were investigated. The molecular weights and the intrinsic viscosity of purified polysaccharide (UM01-S4) were determined as 22,559 Da and 5.09 mL/g, respectively. Using the polymer solution theory, UM01-S4 has a sphere-like conformation in aqueous NaCl at 35 °C. The chemical structure of UM01-S4 contains an α-(1→2)-Manp core. The side chains were composed of β-(1→2)-Galf, β-(1→4)-Glcp, α-GalpA, and α-Manp units, which attached to the mannan core at the O-6 position. The immuno-modulatory assays showed that UM01-S4 exhibited good activities, including macrophages proliferation, phagocytosis, and release of NO and cytokines. The mechanism underlying the macrophage regulation of UM01-S4 might be related to the activation of the MAPK and NF-κB signalling pathways. Thus, mycelium of C. sinensis UM01 could be used as a natural immune-stimulating agent in functional food areas and pharmaceutical industries. |
topic |
Cordyceps sinensis Polysaccharide Chain conformation Chemical structural Immuno-modulatory activity |
url |
http://www.sciencedirect.com/science/article/pii/S1756464616301426 |
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