Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study
chlorpyrifos (CPF) is an organophosphate insecticide used to control pests on a variety of food and feed crops. In mammals, maternal exposure to CPF has been reported to induce cerebral cortex thinning, alteration of long-term brain cognitive function, and Parkinson-like symptoms, but the mechanisms...
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doaj-ffba642a5c084b48810db1ce835633152020-11-24T20:48:16ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-11-011811246710.3390/ijms18112467ijms18112467Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse StudyMaria Michela Pallotta0Raffaele Ronca1Rosa Carotenuto2Immacolata Porreca3Mimmo Turano4Concetta Ambrosino5Teresa Capriglione6Dipartimento di Biologia, Università di Napoli Federico II, Via Cinthia 21, 80126 Napoli, ItalyDipartimento di Biologia, Università di Napoli Federico II, Via Cinthia 21, 80126 Napoli, ItalyDipartimento di Biologia, Università di Napoli Federico II, Via Cinthia 21, 80126 Napoli, ItalyIRGS, Biogem, Via Camporeale, Ariano Irpino, 83031 Avellino, ItalyDipartimento di Biologia, Università di Napoli Federico II, Via Cinthia 21, 80126 Napoli, ItalyIRGS, Biogem, Via Camporeale, Ariano Irpino, 83031 Avellino, ItalyDipartimento di Biologia, Università di Napoli Federico II, Via Cinthia 21, 80126 Napoli, Italychlorpyrifos (CPF) is an organophosphate insecticide used to control pests on a variety of food and feed crops. In mammals, maternal exposure to CPF has been reported to induce cerebral cortex thinning, alteration of long-term brain cognitive function, and Parkinson-like symptoms, but the mechanisms of these processes are not fully understood. In this study, we aimed to gain a deeper understanding of the alterations induced in the brains of mice chronically exposed to CPF by dietary intake. For our purpose, we analysed F1 offspring (sacrificed at 3 and 8 months) of Mus musculus, treated in utero and postnatally with 3 different doses of CPF (0.1-1-10 mg/kg/day). Using RT2 Profiler PCR Arrays, we evaluated the alterations in the expression of 84 genes associated with neurodegenerative diseases. In the brains of exposed mice, we evidenced a clear dose–response relationship for AChE inhibition and alterations of gene expression. Some of the genes that were steadily down-regulated, such as Pink1, Park 2, Sv2b, Gabbr2, Sept5 and Atxn2, were directly related to Parkinson’s onset. Our experimental results shed light on the possibility that long-term CPF exposure may exert membrane signalling alterations which make brain cells more susceptible to develop neurodegenerative diseases.https://www.mdpi.com/1422-0067/18/11/2467chlorpyrifosorganophosphate insecticidesMus musculusdevelopmental neurotoxicityParkinson’s diseaseneurodegenerative diseasesPCR-array |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Michela Pallotta Raffaele Ronca Rosa Carotenuto Immacolata Porreca Mimmo Turano Concetta Ambrosino Teresa Capriglione |
spellingShingle |
Maria Michela Pallotta Raffaele Ronca Rosa Carotenuto Immacolata Porreca Mimmo Turano Concetta Ambrosino Teresa Capriglione Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study International Journal of Molecular Sciences chlorpyrifos organophosphate insecticides Mus musculus developmental neurotoxicity Parkinson’s disease neurodegenerative diseases PCR-array |
author_facet |
Maria Michela Pallotta Raffaele Ronca Rosa Carotenuto Immacolata Porreca Mimmo Turano Concetta Ambrosino Teresa Capriglione |
author_sort |
Maria Michela Pallotta |
title |
Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study |
title_short |
Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study |
title_full |
Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study |
title_fullStr |
Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study |
title_full_unstemmed |
Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study |
title_sort |
specific effects of chronic dietary exposure to chlorpyrifos on brain gene expression—a mouse study |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2017-11-01 |
description |
chlorpyrifos (CPF) is an organophosphate insecticide used to control pests on a variety of food and feed crops. In mammals, maternal exposure to CPF has been reported to induce cerebral cortex thinning, alteration of long-term brain cognitive function, and Parkinson-like symptoms, but the mechanisms of these processes are not fully understood. In this study, we aimed to gain a deeper understanding of the alterations induced in the brains of mice chronically exposed to CPF by dietary intake. For our purpose, we analysed F1 offspring (sacrificed at 3 and 8 months) of Mus musculus, treated in utero and postnatally with 3 different doses of CPF (0.1-1-10 mg/kg/day). Using RT2 Profiler PCR Arrays, we evaluated the alterations in the expression of 84 genes associated with neurodegenerative diseases. In the brains of exposed mice, we evidenced a clear dose–response relationship for AChE inhibition and alterations of gene expression. Some of the genes that were steadily down-regulated, such as Pink1, Park 2, Sv2b, Gabbr2, Sept5 and Atxn2, were directly related to Parkinson’s onset. Our experimental results shed light on the possibility that long-term CPF exposure may exert membrane signalling alterations which make brain cells more susceptible to develop neurodegenerative diseases. |
topic |
chlorpyrifos organophosphate insecticides Mus musculus developmental neurotoxicity Parkinson’s disease neurodegenerative diseases PCR-array |
url |
https://www.mdpi.com/1422-0067/18/11/2467 |
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