Effects of Lingguizhugan Decoction on α-SMA and collagen synthesis in rat myocardial fibroblasts induced by transforming growth factor-β1

• Main Authors: Rui-Rui Ge, Jin-Ling Huang
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2021-05-01
• Series: Journal of Hainan Medical University
• Subjects: lingguizhugan decoction; transforming growth factor-β1; cardiac fibroblasys; myocardial fibrosis; α-sma; collagen; fibronectin
• Online Access: http://www.hnykdxxb.com/PDF/202110/04.pdf

Objective: To explore the protective effect of Linggui Zhugan Decoction (LGZGD) medicated serum on myocardial fibrosis induced by transforming growth factor-β1 (TGF-β1). Methods: Using enzyme digestion method, combined with differential adherence to isolate and culture Sprague-Dawley (SD) suckling mouse Cardiac fibroblasys (CFB) in vitro. Divided into: blank group, blank rat serum group, model group, and LGZGD medicated serum group (5%、10%、20% ). Except for blank group and blank rat serum group, they were stimulated with 5 ng/ml TGF-β1 for 12 hours , and then then intervene with LGZGD medicated serum (5%、10%、20% ) and continue to culture for 24 hours. Use immunofluorescence and Western blot (WB) to detect the expression of α-smooth muscle actin (α-SMA), Enzymelinked immunosorbent assay (ELISA) and WB to detect type I collagen (Collagen I) , type I collagen (Collagen III) and fibronectin (FN) expression. Results: Compared with the blank group, the expressions of Collagen I, Collagen III, α-SMA and FN in the model group were significantly increased (P<0.01); Compared with the model group, the expressions of Collagen I and Collagen III in each concentration group of the experiment were significantly reduced (P<0.01); the expression of α-SMA and FN were significantly reduced (P<0.01). Conclusions: LGZGD has an inhibitory effect on collagen synthesis and the expression of α-SMA and FN, indicating that the anti-fibrosis effect of LGZGD is related to it.