A CRISPR-Cas9 based shuffle system for endogenous histone H3 and H4 combinatorial mutagenesis
Abstract Post-translational modifications of histone proteins greatly impact gene expression and cell fate decisions in eukaryotes. To study these, it is important to develop a convenient, multiplex, and efficient method to precisely introduce mutations to histones. Because eukaryotic cells usually...
Main Authors: | Yu Fu, Zhenglin Zhu, Geng Meng, Rijun Zhang, Yueping Zhang |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-02-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-82774-4 |
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