Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications

Measurement of von Willebrand factor antigen (VWF:Ag) levels is usually performed in a specialised laboratory which limits its application in routine clinical practice. So far, no commercial rapid test kit is available for VWF:Ag estimation. This paper discusses the technical aspect of latex aggluti...

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Bibliographic Details
Main Authors: Mahat, Marianor (Author), Abdullah, Wan Zaidah (Author), Che Hussin, Che Maraina (Author)
Format: Article
Language:English
Published: Hindawi Publishing Corporation, 2014.
Subjects:
Online Access:Get fulltext
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100 1 0 |a Mahat, Marianor  |e author 
700 1 0 |a Abdullah, Wan Zaidah  |e author 
700 1 0 |a Che Hussin, Che Maraina  |e author 
245 0 0 |a Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications 
260 |b Hindawi Publishing Corporation,   |c 2014. 
856 |z Get fulltext  |u http://eprints.usm.my/38530/1/Conventional_Rapid_Latex_Agglutination_in_Estimation_of_von_Willebrand_Factor_Method.pdf 
520 |a Measurement of von Willebrand factor antigen (VWF:Ag) levels is usually performed in a specialised laboratory which limits its application in routine clinical practice. So far, no commercial rapid test kit is available for VWF:Ag estimation. This paper discusses the technical aspect of latex agglutinationmethod which was established to suit the purpose of estimating vonWillebrand factor (VWF) levels in the plasma sample. The latex agglutination test can be performed qualitatively and semiquantitatively. Reproducibility, stability, linearity, limit of detection, interference, and method comparison studies were conducted to evaluate the performance of this test. Semiquantitative latex agglutination test was strongly correlated with the reference immunoturbidimetric assay (Spearman's rho = 0.946, 
546 |a en 
650 0 4 |a QR180-189.5 Immunology