Comparative study of poly(vinyl alcohol)-based support materials for the immobilization of glucose oxidase

BACKGROUND: The performances of four types of glucose oxidase (GOD) immobilization materials based on poly(vinyl alcohol) (PVA) were compared. The matrices of interest were chernically-linked PVA, freeze-thawed PVA cryogel, tetramethoxysilane (TMOS) sol-gel-PVA hybrid material, and alumina sol-gel-P...

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Bibliographic Details
Main Authors: Wong, Fui-Ling (Author), Abdul-Aziz, Azila (Author)
Format: Article
Language:English
Published: John Wiley & Sons Inc., 2008-01.
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Online Access:Get fulltext
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100 1 0 |a Wong, Fui-Ling  |e author 
700 1 0 |a Abdul-Aziz, Azila  |e author 
245 0 0 |a Comparative study of poly(vinyl alcohol)-based support materials for the immobilization of glucose oxidase 
260 |b John Wiley & Sons Inc.,   |c 2008-01. 
856 |z Get fulltext  |u http://eprints.utm.my/id/eprint/12517/1/FuiLingWong2008_JournalofChemicalTechnologyandBiotechnology.pdf 
520 |a BACKGROUND: The performances of four types of glucose oxidase (GOD) immobilization materials based on poly(vinyl alcohol) (PVA) were compared. The matrices of interest were chernically-linked PVA, freeze-thawed PVA cryogel, tetramethoxysilane (TMOS) sol-gel-PVA hybrid material, and alumina sol-gel-PVA hybrid material. RESULTS: Overall, the membranes showed good sensitivity except for the chemically cross-linked PVA. However, the main differences with the enzyme immobilization methods were enzyme leakage and values of K-m(app). CONCLUSION: Freeze-thawed PVA-GOD membranes and TMOS-PVA, which showed satisfactory sensitivity and adequate value of K-m(app), were quite promising as support materials for immobilizing GOD. (c) 2007 Society of Chemical Industry. 
546 |a en 
650 0 4 |a Q Science (General) 
650 0 4 |a QD Chemistry