Rational mutagenesis of cyclodextrin glucanotransferase at the calcium binding regions for enhancement of thermostability
Studies related to the engineering of calcium binding sites of CGTase are limited. The calcium binding regions that are known for thermostability function were subjected to site-directed mutagenesis in this study. The starting gene-protein is a variant of CGTase Bacillus sp. G1, reported earlier and...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI,
2012.
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| Subjects: | |
| Online Access: | Get fulltext |