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01336 am a22001933u 4500 |
| 001 |
5574 |
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|a dc
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|a Ibrahim, Hanan Moawia
|e author
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|a Wan Yusoff, Wan Mokhtar
|e author
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|a Abdul Hamid, Aidil
|e author
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1 |
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|a Md. Illias, Rosli
|e author
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|a Hassan, Othman
|e author
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|a Omar, Othman
|e author
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|a Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD)
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|b Elsevier Limited,
|c 2005.
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| 856 |
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|z Get fulltext
|u http://eprints.utm.my/id/eprint/5574/1/HananMoawiaIbrahim2005_OptimizationOfMedium.pdf
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|a Production of cyclodextrin glucanotransferase (CGTase) from Bacillus G 1, a new bacterial isolate was optimized in shake-flask cultures using statistical design approach. An efficient fermentation medium producing CGTase with high activity (54.9U/ml) was determined: A 2(5) Central Composite Design (CCD; half fraction) has been chosen to elucidate the combined effect of five process variables; tapioca starch, peptone, yeast extract, magnesium sulphate and potassium phosphate concentration. Concentrations of 4% tapioca starch, 2% peptone, 0.04% magnesium sulphate and 1 % sodium carbonate have been shown to be optimum for the production of CGTase.
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|a en
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|a T Technology (General)
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