Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy
High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique c...
Main Authors: | , , , , , , , , , , |
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Other Authors: | , , , , |
Format: | Article |
Language: | English |
Published: |
Springer Nature,
2016-07-08T16:56:10Z.
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Subjects: | |
Online Access: | Get fulltext |
Summary: | High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ~700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging. European Commission (Marie Curie, FP7-PEOPLE-2011-IIF) National Institutes of Health (U.S.) (NIH grant 1R01EY023173) National Science Foundation (U.S.) (NSF CBET 1053233) National Science Foundation (U.S.) (NSF CBET 1344219) National Institutes of Health (U.S.) (NIH 1DP1NS087724) Vienna Science and Technology Fund ((WWTF) project VRG10-11) Seventh Framework Programme (European Commission) (ERC no. 281869) MIT Media Lab Consortium Institution of Engineering and Technology (IET) (Harvey Prize) Human Frontier Science Program (Strasbourg, France) (Project RGP0041/2012) |
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