Characterization of a genetic and antigenic variant of avian paramyxovirus 6 isolated from a migratory wild bird, the red-necked stint (Calidris ruficollis)

• Main Authors: Bui, Vuong Nghia (Author), Mizutani, Tetsuya (Author), Nguyen, Tung Hoang (Author), Trinh, Dai Quang (Author), Awad, Sanaa S. A. (Author), Minoungou, Germaine L. (Author), Yamamoto, Yu (Author), Nakamura, Kikuyasu (Author), Saito, Keisuke (Author), Watanabe, Yukiko (Author), Runstadler, Jonathan (Contributor), Huettmann, Falk (Author), Ogawa, Haruko (Author), Imai, Kunitoshi (Author)
• Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor)
• Format: Article
• Language: English
• Published: Springer Vienna, 2016-09-22T17:56:58Z.
• Subjects: Article
• Online Access: Get fulltext

A hemagglutinating virus (8KS0813) was isolated from a red-necked stint. Hemagglutination inhibition and neutralization tests indicated that 8KS0813 was antigenically related to a prototype strain, APMV-6/duck/Hong Kong/18/199/77, but with an 8- and 16-fold difference, respectively, in their titers. The full genome sequence of 8KS0813 showed 98.6 % nucleotide sequence identity to that of APMV-6/duck/Italy/4524-2/07, which has been reported to belong to an APMV-6 subgroup, and showed less similarity to that of the prototype strain (70.6 % similarity). The growth of 8KS0813 and the prototype strain in four different cell cultures was greatly enhanced by adding trypsin. Interestingly, this virus induced syncytia only in Vero cells. 8KS0813 was identified as APMV-6/red-necked stint/Japan/8KS0813/08, but it is antigenically and genetically distinguishable from the prototype strain, suggesting that variant APMV-6 is circulating in migratory birds.
Program of Founding Research Centers for Emerging and Reemerging Infectious Diseases
Japan. Ministry of Education, Culture, Sports, Science and Technology (Grant-in-Aid for Exploratory Research (19659115))
National Institute of Allergy and Infectious Diseases (U.S.) (NIAID contracts HHSN266200700009C and HHSN266200700007C)