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|a Bai, Zhiqiang
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|a Massachusetts Institute of Technology. Department of Biology
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|a Whitehead Institute for Biomedical Research
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|a Alvarez, Juan Rene
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|a Slavov, Nikolai G
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|a Lodish, Harvey F
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|a Xu, Dan
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|a Yuan, Bingbing
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|a Lo, Kinyui Alice
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|a Yoon, Myeong Jin
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|a Lim, Yen Ching
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|a Knoll, Marko
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|a Chen, Shuai
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|a Chen, Peng
|e author
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|a Sun, Lei
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|a Alvarez, Juan Rene
|e author
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|a Slavov, Nikolai G
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|a Lodish, Harvey F
|e author
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|a De Novo Reconstruction of Adipose Tissue Transcriptomes Reveals Long Non-coding RNA Regulators of Brown Adipocyte Development
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|b Elsevier,
|c 2016-12-13T15:00:06Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/105804
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|a Brown adipose tissue (BAT) protects against obesity by promoting energy expenditure via uncoupled respiration. To uncover BAT-specific long non-coding RNAs (lncRNAs), we used RNA-seq to reconstruct de novo transcriptomes of mouse brown, inguinal white, and epididymal white fat and identified ∼1,500 lncRNAs, including 127 BAT-restricted loci induced during differentiation and often targeted by key regulators PPARγ, C/EBPα, and C/EBPβ. One of them, lnc-BATE1, is required for establishment and maintenance of BAT identity and thermogenic capacity. lnc-BATE1 inhibition impairs concurrent activation of brown fat and repression of white fat genes and is partially rescued by exogenous lnc-BATE1 with mutated siRNA-targeting sites, demonstrating a function in trans. We show that lnc-BATE1 binds heterogeneous nuclear ribonucleoprotein U and that both are required for brown adipogenesis. Our work provides an annotated catalog for the study of fat depot-selective lncRNAs and establishes lnc-BATE1 as a regulator of BAT development and physiology.
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|a National Institutes of Health (U.S.) (Grants DK047618, DK068348 and 5P01 HL066105)
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|a Singapore. Ministry of Health (Singapore National Research Foundation. CBRG Grant NMRC/CBRG/0070/2014)
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|a en_US
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|a Article
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|t Cell Metabolism
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