TRIB2 reinforces the oncogenic transcriptional program controlled by the TAL1 complex in T-cell acute lymphoblastic leukemia
T-cell acute lymphoblastic leukemia (T-ALL) is a malignant disorder resulting from the leukemic transformation of T-cell precursors, and is one of the most common forms of cancer in children and is also found in adults. The most frequent genetic abnormality in T-ALL is the dysregulation of transcrip...
Main Authors: | , , , , , , , |
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Other Authors: | , , |
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group,
2017-02-10T16:09:48Z.
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Online Access: | Get fulltext |
Summary: | T-cell acute lymphoblastic leukemia (T-ALL) is a malignant disorder resulting from the leukemic transformation of T-cell precursors, and is one of the most common forms of cancer in children and is also found in adults. The most frequent genetic abnormality in T-ALL is the dysregulation of transcription factor genes, including aberrant expression of TAL1/SCL. TAL1 is ectopically expressed in 40-60% of T-ALL cases owing to chromosomal translocation, intrachromosomal rearrangement ('SIL-TAL deletion') or a somatic mutation in a non-coding intergenic element. We previously reported that TAL1 forms a positive interconnected autoregulatory loop with its regulatory partners GATA3, RUNX1 and MYB ('core regulatory circuits'). To identify critical downstream targets that contribute to T-cell leukemogenesis, we have also performed a loss-of-function RNA interference screen in the TAL1-positive T-ALL cell lines in our previous study.6 Among the high-confidence TAL1 targets, only four genes, including the Tribbles homolog 2 (TRIB2), were selected by this screen. TRIB2 is a pseudo-kinase protein and has been implicated as an oncogene that contributes to acute myeloid leukemia (AML) by negatively regulating the C/EBPα tumor suppressor protein. TRIB2 is also a transcriptional target of the oncogene NOTCH1 in T-ALL. However, the oncogenic role of TRIB2 in T-ALL pathogenesis, in particular, as a downstream consequence of the core regulatory circuits, has not been elucidated. In this study, we identified molecular pathways regulated by TRIB2 in T-ALL cells. National Cancer Institute (U.S.) (Grants 1K99CA157951 and 5P01CA109901) Singapore. National Research Foundation. Competitive Research Programme (Award NRF-NRFF2013-02) |
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