Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device

• Main Authors: Rowlands, Christopher (Author), Park, Jong Kang (Contributor), So, Peter T. C. (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Laser Biomedical Research Center (Contributor)
• Format: Article
• Language: English
• Published: MDPI AG, 2017-06-22T14:49:18Z.
• Subjects: Article
• Online Access: Get fulltext

Temporal focusing multiphoton microscopy is a technique for performing highly parallelized multiphoton microscopy while still maintaining depth discrimination. While the conventional wide-field configuration for temporal focusing suffers from sub-optimal axial resolution, line scanning temporal focusing, implemented here using a digital micromirror device (DMD), can provide substantial improvement. The DMD-based line scanning temporal focusing technique dynamically trades off the degree of parallelization, and hence imaging speed, for axial resolution, allowing performance parameters to be adapted to the experimental requirements. We demonstrate this new instrument in calibration specimens and in biological specimens, including a mouse kidney slice.
United States. National Institutes of Health (5-P41-EB015871-28)
United States. National Institutes of Health (2R01EY017656-06)
United States. National Institutes of Health (R21-NS091982-01)
United States. National Institutes of Health (1-U01-NS090438-01)
United States. National Institutes of Health (1U01CA202177-01)
United States. National Institutes of Health (1R01HL121386-01A1)