Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays

Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays

Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however...

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Bibliographic Details
Main Authors: Carter, Bradley S. (Author), Cortés-Campos, Christian (Author), McCammon, Jasmine M. (Author), Chen, Xiao (Contributor), Sive, Hazel L. (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Sive, Hazel L (Contributor)
Format: Article
Language:English
Published: Mary Ann Liebert, Inc, 2017-09-07T17:34:30Z.
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Summary:Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however, this approach requires target-specific antibodies that are generally not available for zebrafish proteins. We address this issue using in vitro translation assays, a fast and standard procedure that can be easily implemented.
National Science Foundation (U.S.) (IOS-1258087)

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