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|a Fukumura, Dai
|e author
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|a Massachusetts Institute of Technology. Department of Biological Engineering
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|a Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
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|a Massachusetts Institute of Technology. Department of Chemistry
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|a Massachusetts Institute of Technology. Department of Mechanical Engineering
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|a Massachusetts Institute of Technology. Media Laboratory
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|a McGovern Institute for Brain Research at MIT
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|a Massachusetts Institute of Technology. Center for Neurobiological Engineering
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|a Rowlands, Christopher
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|a Park, Demian
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|a Bruns, Oliver Thomas
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|a Piatkevich, Kiryl
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|a Bawendi, Moungi G
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|a Boyden, Edward
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|a So, Peter T. C.
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|a Jain, Rakesh K
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|a Rowlands, Christopher
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|a Park, Demian
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|a Bruns, Oliver Thomas
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|a Piatkevich, Kiryl
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|a Bawendi, Moungi G
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|a Boyden, Edward
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|a So, Peter T. C.
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|a Wide-field three-photon excitation in biological samples
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|b Springer Nature,
|c 2018-01-08T17:03:06Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/113016
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|a Three-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy. Excitation of chromophores as diverse as channelrhodopsins and quantum dots is shown, and a penetration depth of more than 700 μm into fixed scattering brain tissue is achieved, approximately twice as deep as that achieved using two-photon wide-field excitation. Compatibility with live animal experiments is confirmed by imaging the cerebral vasculature of an anesthetized mouse; a complete focal stack was obtained without any evidence of photodamage. As an additional validation of the utility of wide-field three-photon excitation, functional excitation is demonstrated by performing three-photon optogenetic stimulation of cultured mouse hippocampal neurons expressing a channelrhodopsin; action potentials could reliably be excited without causing photodamage.
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|a National Institutes of Health (U.S.) (Grant NIH-5-P41-EB015871-27)
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|a National Institutes of Health (U.S.) (Grant DP3-DK101024 01)
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|a National Institutes of Health (U.S.) (Grant 1-U01-NS090438-01)
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|a National Institutes of Health (U.S.) (1-R01-HL121386-01A1)
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|a National Institutes of Health (U.S.) (Grant 1-R01-EY017656-06A1)
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|a National Institutes of Health (U.S.) (Grant 1R24MH106075)
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|a National Institutes of Health (U.S.) (Grant 2R01DA029639)
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|a National Institutes of Health (U.S.) (Grant 1R01MH103910)
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|a National Institutes of Health (U.S.) (Grant 1R01GM104948)
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|a National Science Foundation (U.S.) (Grant CBET 1053233)
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|a National Institutes of Health (U.S.) (Grant 5U54 CA151884-04)
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|a National Institutes of Health (U.S.) (Grant 9-P41-EB015871-26A1)
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|a Article
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|t Light: Science & Applications
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