A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response

A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response

Functional genomics efforts face tradeoffs between number of perturbations examined and complexity of phenotypes measured. We bridge this gap with Perturb-seq, which combines droplet-based single-cell RNA-seq with a strategy for barcoding CRISPR-mediated perturbations, allowing many perturbations to...

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Bibliographic Details
Main Authors: Adamson, Britt (Author), Norman, Thomas M. (Author), Jost, Marco (Author), Cho, Min Y. (Author), Nuñez, James K. (Author), Chen, Yuwen (Author), Villalta, Jacqueline E. (Author), Gilbert, Luke A. (Author), Horlbeck, Max A. (Author), Hein, Marco Y. (Author), Pak, Ryan A. (Author), Gray, Andrew N. (Author), Gross, Carol A. (Author), Parnas, Oren (Author), Weissman, Jonathan S. (Author), Dixit, Atray C. (Contributor), Regev, Aviv (Contributor)
Other Authors: Institute for Medical Engineering and Science (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor)
Format: Article
Language:English
Published: Elsevier, 2018-07-03T17:35:18Z.
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