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|a dc
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|a Lummertz da Rocha, Edroaldo
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|a Massachusetts Institute of Technology. Institute for Medical Engineering & Science
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|a Massachusetts Institute of Technology. Department of Biological Engineering
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|a Massachusetts Institute of Technology. Synthetic Biology Center
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|a Collins, James J.
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|a Rowe, R. Grant
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|a Lundin, Vanessa
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|a Malleshaiah, Mohan
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|a Jha, Deepak Kumar
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|a Rambo, Carlos R.
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|a Li, Hu
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|a North, Trista E.
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|a Collins, James J.
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|a Daley, George Q.
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|a Reconstruction of complex single-cell trajectories using CellRouter
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|b Nature Publishing Group,
|c 2018-08-28T16:17:04Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/117589
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|a A better understanding of the cell-fate transitions that occur in complex cellular ecosystems in normal development and disease could inform cell engineering efforts and lead to improved therapies. However, a major challenge is to simultaneously identify new cell states, and their transitions, to elucidate the gene expression dynamics governing cell-type diversification. Here, we present CellRouter, a multifaceted single-cell analysis platform that identifies complex cell-state transition trajectories by using flow networks to explore the subpopulation structure of multi-dimensional, single-cell omics data. We demonstrate its versatility by applying CellRouter to single-cell RNA sequencing data sets to reconstruct cell-state transition trajectories during hematopoietic stem and progenitor cell (HSPC) differentiation to the erythroid, myeloid and lymphoid lineages, as well as during re-specification of cell identity by cellular reprogramming of monocytes and B-cells to HSPCs. CellRouter opens previously undescribed paths for in-depth characterization of complex cellular ecosystems and establishment of enhanced cell engineering approaches.
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|a National Institute of Diabetes and Digestive and Kidney Diseases (U.S.) (Grant R24DK092760)
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|a National Institute of Allergy and Infectious Diseases (U.S.) (Grant R37AI039394)
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|a National Heart, Lung, and Blood Institute (Grant UO1-HL100001)
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|a Article
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|t Nature Communications
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