Engineering Engineering Hyperthermostable rcSso7d as Reporter Molecule for in Vitro Diagnostic Tests

• Main Authors: Sung, Ki-Joo (Contributor), Miller, Eric Alexander (Contributor), Sikes Johnson, Hadley (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor)
• Format: Article
• Language: English
• Published: Royal Society of Chemistry (RSC), 2018-11-19T19:35:53Z.
• Subjects: Article
• Online Access: Get fulltext

The hyperthermostable binding scaffold, rcSso7d, has recently been studied for use in in vitro diagnostic tests but has not yet been demonstrated as a soluble reporter protein. Here, we investigated several rcSso7d protein constructs and biotin conjugation methods to link a detectable signal to the presence of a target biomarker.
National Science Foundation (U.S.) (Grant 1122374)