foxF-1 Controls Specification of Non-body Wall Muscle and Phagocytic Cells in Planarians

Planarians are flatworms capable of regenerating any missing body part in a process requiring stem cells and positional information. Muscle is a major source of planarian positional information and consists of several types of fibers with distinct regulatory roles in regeneration. The transcriptiona...

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Main Authors: Scimone, M. Lucila (Author), Wurtzel, Omri (Author), Malecek, Kathryn (Author), Fincher, Christopher T. (Author), Oderberg, Isaac M. (Author), Kravarik, Kellie M. (Author), Reddien, Peter W. (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Whitehead Institute for Biomedical Research (Contributor)
Format: Article
Language:English
Published: Elsevier BV, 2020-05-12T13:38:49Z.
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Online Access:Get fulltext
LEADER 02743 am a22002653u 4500
001 125172
042 |a dc 
100 1 0 |a Scimone, M. Lucila  |e author 
100 1 0 |a Massachusetts Institute of Technology. Department of Biology  |e contributor 
100 1 0 |a Whitehead Institute for Biomedical Research  |e contributor 
700 1 0 |a Wurtzel, Omri  |e author 
700 1 0 |a Malecek, Kathryn  |e author 
700 1 0 |a Fincher, Christopher T.  |e author 
700 1 0 |a Oderberg, Isaac M.  |e author 
700 1 0 |a Kravarik, Kellie M.  |e author 
700 1 0 |a Reddien, Peter W.  |e author 
245 0 0 |a foxF-1 Controls Specification of Non-body Wall Muscle and Phagocytic Cells in Planarians 
260 |b Elsevier BV,   |c 2020-05-12T13:38:49Z. 
856 |z Get fulltext  |u https://hdl.handle.net/1721.1/125172 
520 |a Planarians are flatworms capable of regenerating any missing body part in a process requiring stem cells and positional information. Muscle is a major source of planarian positional information and consists of several types of fibers with distinct regulatory roles in regeneration. The transcriptional regulatory programs used to specify different muscle fibers are poorly characterized. Using single-cell RNA sequencing, we define the transcriptomes of planarian dorsal-ventral muscle (DVM), intestinal muscle (IM), and pharynx muscle. This analysis identifies foxF-1, which encodes a broadly conserved Fox-family transcription factor, as a master transcriptional regulator of all non-body wall muscle. The transcription factors encoded by nk4 and gata4/5/6-2 specify two different subsets of DVM, lateral and medial, respectively, whereas gata4/5/6-3 specifies IM. These muscle types all express planarian patterning genes. Both lateral and medial DVM are required for medial-lateral patterning in regeneration, whereas medial DVM and IM have a role in maintaining and regenerating intestine morphology. In addition to the role in muscle, foxF-1 is required for the specification of multiple cell types with transcriptome similarities, including high expression levels of cathepsin genes. These cells include pigment cells, glia, and several other cells with unknown function. cathepsin+ cells phagocytose E. coli, suggesting these are phagocytic cells. In conclusion, we describe a regulatory program for planarian muscle cell subsets and phagocytic cells, both driven by foxF-1. FoxF proteins specify different mesoderm-derived tissues in other organisms, suggesting that FoxF regulates formation of an ancient and broadly conserved subset of mesoderm derivatives in the Bilateria. 
520 |a National Institutes of Health (U.S.) (Grant R01GM080639) 
546 |a en 
655 7 |a Article 
773 |t 10.1016/J.CUB.2018.10.030 
773 |t Current biology