CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses
A key goal for cell biological analyses is to assess the phenotypes that result from eliminating a target gene. Since the early 1990s, the predominant strategy utilized in human tissue culture cells has been RNA interference (RNAi)-mediated protein depletion. However, RNAi suffers well-documented of...
Main Authors: | , , , |
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Other Authors: | |
Format: | Article |
Language: | English |
Published: |
American Society for Cell Biology (ASCB),
2020-07-10T20:38:30Z.
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Subjects: | |
Online Access: | Get fulltext |