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|a Ohayon, Shay
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|a McGovern Institute for Brain Research at MIT
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|a Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
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|a DiCarlo, James
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|a Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging
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|b The Optical Society,
|c 2020-08-20T11:16:55Z.
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|z Get fulltext
|u https://hdl.handle.net/1721.1/126696
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|a A major open challenge in neuroscience is the ability to measure and perturb neural activity in vivo from well defined neural sub-populations at cellular resolution anywhere in the brain. However, limitations posed by scattering and absorption prohibit non-invasive multi-photon approaches for deep (>2mm) structures, while gradient refractive index (GRIN) endoscopes are relatively thick and can cause significant damage upon insertion. Here, we present a novel micro-endoscope design to image neural activity at arbitrary depths via an ultra-thin multi-mode optical fiber (MMF) probe that has 5-10X thinner diameter than commercially available microendoscopes. We demonstrate micron-scale resolution, multi-spectral and volumetric imaging. In contrast to previous approaches, we show that this method has an improved acquisition speed that is sufficient to capture rapid neuronal dynamics in-vivo in rodents expressing a genetically encoded calcium indicator (GCaMP). Our results emphasize the potential of this technology in neuroscience applications and open up possibilities for cellular resolution imaging in previously unreachable brain regions.
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|a National Institutes of Health (U.S.) (Grant (REY026436A)
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|a Article
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|t 10.1364/BOE.9.001492
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|t Biomedical optics express
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