Optical Pooled Screens in Human Cells

• Main Authors: Feldman, David (Author), Singh, Avtar (Author), Schmid-Burgk, Jonathan (Author), Carlson, Rebecca J. (Author), Mezger, Anja (Author), Garrity, Anthony J. (Author), Zhang, Feng (Author), Blainey, Paul C (Author)
• Other Authors: Massachusetts Institute of Technology. Department of Physics (Contributor), Harvard University- (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), McGovern Institute for Brain Research at MIT (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), Broad Institute of MIT and Harvard (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor)
• Format: Article
• Language: English
• Published: Elsevier BV, 2020-10-21T21:09:19Z.
• Subjects: Article
• Online Access: Get fulltext

A screening approach that combines high-content imaging with in situ sequencing can identify genes that affect spatially and temporally defined phenotypes like morphology and subcellular localization, expanding the list of scientific questions that can be asked with genetic tools.
National Human Genome Research Institute (Grants HG009283 and HG006193)
NIH (Grants 1R01-HG009761, 1R01-MH110049, and 1DP1-HL141201)