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|a Yoon, Young-Gyu
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|a Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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|a Massachusetts Institute of Technology. Center for Neurobiological Engineering
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|a Massachusetts Institute of Technology. Department of Mechanical Engineering
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|a Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
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|a Massachusetts Institute of Technology. Department of Biological Engineering
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|a McGovern Institute for Brain Research at MIT
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|a Program in Media Arts and Sciences
|q (Massachusetts Institute of Technology)
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|a Koch Institute for Integrative Cancer Research at MIT
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|a Wang, Zeguan
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|a Pak, Nikita
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|a Park, Demian
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|a Dai, Peilun
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|a Kang, Jeong Seuk
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|a Suk, Ho-Jun
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|a Symvoulidis, Panagiotis
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|a Guner-Ataman, Burcu
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|a Wang, Kai
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|a Boyden, Edward
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|a Sparse decomposition light-field microscopy for high speed imaging of neuronal activity
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|b The Optical Society,
|c 2021-03-29T14:37:35Z.
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|z Get fulltext
|u https://hdl.handle.net/1721.1/130251
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|a One of the major challenges in large scale optical imaging of neuronal activity is to simultaneously achieve sufficient temporal and spatial resolution across a large volume. Here, we introduce sparse decomposition light-field microscopy (SDLFM), a computational imaging technique based on light-field microscopy (LFM) that takes algorithmic advantage of the high temporal resolution of LFM and the inherent temporal sparsity of spikes to improve effective spatial resolution and signal-to-noise ratios (SNRs). With increased effective spatial resolution and SNRs, neuronal activity at the single-cell level can be recovered over a large volume. We demonstrate the single-cell imaging capability of SDLFM with in vivo imaging of neuronal activity of whole brains of larval zebrafish with estimated lateral and axial resolutions of ∼3.5 µm and ∼7.4 µm, respectively, acquired at volumetric imaging rates up to 50 Hz. We also show that SDLFM increases the quality of neural imaging in adult fruit flies.
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|a National Science Foundation (Grant 1848029)
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|a U. S. Army Research Laboratory and the U. S. Army Research Office (Contract W911NF1510548)
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|a National Institutes of Health (Grants 1R01DA045549, 1R41MH112318, 1R43MH109332, 1RM1HG008525, 1DP1NS087724)
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|a en
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|a Article
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|t Optica
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