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|a dc
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|a Li, Yinqing
|e author
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|a McGovern Institute for Brain Research at MIT
|e contributor
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|a Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
|e contributor
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|a Klarman Cell Observatory
|q (Broad Institute)
|e contributor
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|a Lopez Huerta, Violeta
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|a Adiconis, Xian
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|a Levandowski, Kirsten
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|a Choi, Soonwook
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|a Simmons, Sean K.
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|a Arias-Garcia, Mario A.
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|a Guo, Baolin
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|a Yao, Annie
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|a Blosser, Timothy R.
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|a Wimmer, Ralf D
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|a Aida, Tomomi
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|a Atamian, Alexander
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|a Naik, Tina
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|a Sun, Xuyun
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|a Bi, Dasheng
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|a Malhotra, Diya
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|a Hession, Cynthia C.
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|a Shema Tirosh, Reut
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|a Gomes, Marcos
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|a Li, Taibo
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|a Hwang, Eunjin
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|a Krol, Alexandra
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|a Kowalczyk, Monika
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|a Peça, João
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|a Pan, Gang
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|a Halassa, Michael
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|a Levin, Joshua Z.
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|a Fu, Zhanyan
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|a Feng, Guoping
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|a Distinct subnetworks of the thalamic reticular nucleus
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|b Springer Science and Business Media LLC,
|c 2021-04-07T21:02:09Z.
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|z Get fulltext
|u https://hdl.handle.net/1721.1/130405
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|a The thalamic reticular nucleus (TRN), the major source of thalamic inhibition, regulates thalamocortical interactions that are critical for sensory processing, attention and cognition1-5. TRN dysfunction has been linked to sensory abnormality, attention deficit and sleep disturbance across multiple neurodevelopmental disorders6-9. However, little is known about the organizational principles that underlie its divergent functions. Here we performed an integrative study linking single-cell molecular and electrophysiological features of the mouse TRN to connectivity and systems-level function. We found that cellular heterogeneity in the TRN is characterized by a transcriptomic gradient of two negatively correlated gene-expression profiles, each containing hundreds of genes. Neurons in the extremes of this transcriptomic gradient express mutually exclusive markers, exhibit core or shell-like anatomical structure and have distinct electrophysiological properties. The two TRN subpopulations make differential connections with the functionally distinct first-order and higher-order thalamic nuclei to form molecularly defined TRN-thalamus subnetworks. Selective perturbation of the two subnetworks in vivo revealed their differential role in regulating sleep. In sum, our study provides a comprehensive atlas of TRN neurons at single-cell resolution and links molecularly defined subnetworks to the functional organization of thalamocortical circuits.
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|a NIH/NIMH (Grants R01NS098505, R01NS113245)
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|a NIH (Grants R01NS098505, R01MH107680)
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|a en
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|a Article
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|t Nature
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