Immuno-Hybridization Chain Reaction for Enhancing Detection of Individual Cytokine-Secreting Human Peripheral Mononuclear Cells

We present here a new method to enhance the detection of secreted cytokines and chemokines from single human mononuclear cells. The technique uses a hybridization chain reaction (HCR) to amplify signals resulting from sandwich immunoassays. This immuno-HCR employs oligonucleotide-based initiators co...

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Bibliographic Details
Main Authors: Choi, Jonghoon (Author), Routenberg Love, Kerry (Author), Gong, Yuan (Author), Gierahn, Todd M (Author), Love, J Christopher (Author)
Format: Article
Language:English
Published: American Chemical Society (ACS), 2021-10-27T19:57:15Z.
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