| Summary: | <jats:p>Blue iguanas (<jats:italic>Cyclura lewisi</jats:italic>) are endangered reptiles found only on Grand Cayman. Previously, DNA for a novel <jats:italic>Helicobacter</jats:italic> species GCBI1 was detected in sick and dead iguanas. In the current study, fecal and cloacal swab samples were obtained from 25 iguanas. Through molecular and microbiological techniques, a novel <jats:italic>Helicobacter</jats:italic> species was cultured from feces and characterized, for whom we propose the name <jats:italic>Helicobacter cyclurae</jats:italic>. This novel helicobacter had a prevalence of 56% by PCR and 20% by culture in samples analyzed. The type strain MIT 16-1353 was catalase, oxidase, and gamma-glutamyl transpeptidase positive. By electron microscopy, <jats:italic>H. cyclurae</jats:italic> has a curved rod morphology and a single sheathed polar flagellum. Phylogenetic analysis using 16S rRNA, <jats:italic>gyr</jats:italic>B, and <jats:italic>hsp</jats:italic>60 indicated that these strains were most closely related to <jats:italic>Helicobacter</jats:italic> sp. 12502256-12 previously isolated from lizards. <jats:italic>H. cyclurae</jats:italic> has a 1.91-Mb genome with a GC content of 33.37%. There were 1,969 genes with four notable virulence genes: high temperature requirement-A protein-secreted serine protease, gamma-glutamyl transpeptidase, fibronectin/fibrinogen binding protein, and neutrophil-activating protein. Whole-genome phylogeny, average nucleotide identity, and digital DNA-DNA hybridization analysis confirmed that <jats:italic>H. cyclurae</jats:italic> is a novel species, and the first helicobacter cultured and characterized from blue iguanas.</jats:p>
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