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|a Banigan, Edward J
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|a Mirny, Leonid A
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|a The interplay between asymmetric and symmetric DNA loop extrusion
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|b eLife Sciences Publications, Ltd,
|c 2021-12-09T13:20:17Z.
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|z Get fulltext
|u https://hdl.handle.net/1721.1/138403
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|a © Banigan and Mirny. Chromosome compaction is essential for reliable transmission of genetic information. Experiments suggest that -1000-fold compaction is driven by condensin complexes that extrude chromatin loops, by progressively collecting chromatin fiber from one or both sides of the complex to form a growing loop. Theory indicates that symmetric two-sided loop extrusion can achieve such compaction, but recent single-molecule studies (Golfier et al., 2020) observed diverse dynamics of condensins that perform one-sided, symmetric two-sided, and asymmetric two-sided extrusion. We use simulations and theory to determine how these molecular properties lead to chromosome compaction. High compaction can be achieved if even a small fraction of condensins have two essential properties: A long residence time and the ability to perform two-sided (not necessarily symmetric) extrusion. In mixtures of condensins I and II, coupling two-sided extrusion and stable chromatin binding by condensin II promotes compaction. These results provide missing connections between single-molecule observations and chromosome-scale organization.
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|a en
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|a Article
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|t 10.7554/ELIFE.63528
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|t eLife
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