CRISPR/Cas9 editing to generate a heterozygous COL2A1 p.G1170S human chondrodysplasia iPSC line, MCRIi019-A-2, in a control iPSC line, MCRIi019-A

© 2020 To develop an in vitro disease model of a human chondrodysplasia, we used CRISPR/Cas9 gene editing to generate a heterozygous COL2A1 exon 50 c.3508 GGT > TCA (p.G1170S) mutation in a control human iPSC line. Both the control and COL2A1 mutant lines displayed typical iPSC characteristics, i...

Full description

Bibliographic Details
Main Authors: Kung, Louise HW (Author), Sampurno, Lisa (Author), Yammine, Kathryn M (Author), Graham, Alison (Author), McDonald, Penny (Author), Bateman, John F (Author), Shoulders, Matthew D (Author), Lamandé, Shireen R (Author)
Format: Article
Language:English
Published: Elsevier BV, 2022-03-18T18:50:29Z.
Subjects:
Online Access:Get fulltext