Postsynaptic regulation of synaptic plasticity by synaptotagmin 4 requires both C2 domains

• Main Authors: Littleton, J. Troy (Contributor), Melom, Jan Elizabeth (Contributor), Jorquera, Ramon (Contributor), Barber, Cynthia F. (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), Picower Institute for Learning and Memory (Contributor)
• Format: Article
• Language: English
• Published: The Rockefeller University Press, 2010-04-14T18:02:49Z.
• Subjects: Article
• Online Access: Get fulltext

Ca[superscript 2+] influx into synaptic compartments during activity is a key mediator of neuronal plasticity. Although the role of presynaptic Ca[superscript 2+] in triggering vesicle fusion though the Ca[superscript 2+] sensor synaptotagmin 1 (Syt 1) is established, molecular mechanisms that underlie responses to postsynaptic Ca[superscript 2+] influx remain unclear. In this study, we demonstrate that fusion-competent Syt 4 vesicles localize postsynaptically at both neuromuscular junctions (NMJs) and central nervous system synapses in Drosophila melanogaster. Syt 4 messenger RNA and protein expression are strongly regulated by neuronal activity, whereas altered levels of postsynaptic Syt 4 modify synaptic growth and presynaptic release properties. Syt 4 is required for known forms of activity-dependent structural plasticity at NMJs. Synaptic proliferation and retrograde signaling mediated by Syt 4 requires functional C2A and C2B Ca[superscript 2+]-binding sites, as well as serine 284, an evolutionarily conserved substitution for a key Ca[superscript 2+]-binding aspartic acid found in other synaptotagmins. These data suggest that Syt 4 regulates activity-dependent release of postsynaptic retrograde signals that promote synaptic plasticity, similar to the role of Syt 1 as a Ca[superscript 2+] sensor for presynaptic vesicle fusion.
United States National Institutes of Health (Grant NS40296)