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|a dc
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|a McCarren, Jay
|e author
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|a Massachusetts Institute of Technology. Department of Biological Engineering
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|a Massachusetts Institute of Technology. Department of Biology
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|a Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
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|a DeLong, Edward
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|a McCarren, Jay
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|a Becker, Jamie William
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|a Shi, Yanmei
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|a Young, Curtis Robert, III
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|a Malmstrom, Rex R.
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|a Chisholm, Sallie
|q (Penny)
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|a DeLong, Edward
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|a Becker, Jamie William
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|a Repeta, Daniel J.
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|a Shi, Yanmei
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|a Young, Curtis Robert, III
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|a Malmstrom, Rex R.
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|a Chisholm, Sallie
|q (Penny)
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|a DeLong, Edward Francis
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|a Microbial community transcriptomes reveal microbes and metabolic pathways associated with dissolved organic matter turnover in the sea
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|b National Academy of Sciences,
|c 2011-07-15T14:49:53Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/64819
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|a Marine dissolved organic matter (DOM) contains as much carbon as the Earth's atmosphere, and represents a critical component of the global carbon cycle. To better define microbial processes and activities associated with marine DOM cycling, we analyzed genomic and transcriptional responses of microbial communities to high-molecular-weight DOM (HMWDOM) addition. The cell density in the unamended control remained constant, with very few transcript categories exhibiting significant differences over time. In contrast, the DOM-amended microcosm doubled in cell numbers over 27 h, and a variety of HMWDOM-stimulated transcripts from different taxa were observed at all time points measured relative to the control. Transcripts significantly enriched in the HMWDOM treatment included those associated with two-component sensor systems, phosphate and nitrogen assimilation, chemotaxis, and motility. Transcripts from Idiomarina and Alteromonas spp., the most highly represented taxa at the early time points, included those encoding TonB-associated transporters, nitrogen assimilation genes, fatty acid catabolism genes, and TCA cycle enzymes. At the final time point, Methylophaga rRNA and non-rRNA transcripts dominated the HMWDOM-amended microcosm, and included gene transcripts associated with both assimilatory and dissimilatory single-carbon compound utilization. The data indicated specific resource partitioning of DOM by different bacterial species, which results in a temporal succession of taxa, metabolic pathways, and chemical transformations associated with HMWDOM turnover. These findings suggest that coordinated, cooperative activities of a variety of bacterial "specialists" may be critical in the cycling of marine DOM, emphasizing the importance of microbial community dynamics in the global carbon cycle.
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|a United States. Dept. of Energy
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|a National Science Foundation (U.S.)
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|a National Science Foundation (U.S.) (Science and Technology Center Award EF0424599)
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|a Gordon and Betty Moore Foundation
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|a en_US
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|a Article
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|t Proceedings of the National Academy of Sciences of the United States of America
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