The Effect of Self-Assembling Peptide RADA16-I on the Growth of Human Leukemia Cells in Vitro and in Nude Mice

• Main Authors: Tang, Chenkang (Author), Shao, Ximing (Author), Sun, Binbin (Author), Huang, Wenli (Author), Zhao, Xiaojun (Contributor)
• Other Authors: Massachusetts Institute of Technology. Center for Biomedical Engineering (Contributor)
• Format: Article
• Language: English
• Published: Molecular Diversity Preservation International, 2011-08-15T17:44:36Z.
• Subjects: Article
• Online Access: Get fulltext

Nanofiber scaffolds formed by self-assembling peptide RADA16-I have been used for the study of cell proliferation to mimic an extracellular matrix. In this study, we investigated the effect of RADA16-I on the growth of human leukemia cells in vitro and in nude mice. Self-assembly assessment showed that RADA16-I molecules have excellent self-assembling ability to form stable nanofibers. MTT assay displayed that RADA16-I has no cytotoxicity for leukemia cells and human umbilical vein endothelial cells (HUVECs) in vitro. However, RADA16-I inhibited the growth of K562 tumors in nude mice. Furthermore, we found RADA16-I inhibited vascular tube-formation by HUVECs in vitro. Our data suggested that nanofiber scaffolds formed by RADA16-I could change tumor microenvironments, and inhibit the growth of tumors. The study helps to encourage further design of self-assembling systems for cancer therapy.
China. Ministry of Education (project 985)