A Robust and Rapid Method of Producing Soluble, Stable, and Functional G-Protein Coupled Receptors

A Robust and Rapid Method of Producing Soluble, Stable, and Functional G-Protein Coupled Receptors

Membrane proteins, particularly G-protein coupled receptors (GPCRs), are notoriously difficult to express. Using commercial E.coli cell-free systems with the detergent Brij-35, we could rapidly produce milligram quantities of 13 unique GPCRs. Immunoaffinity purification yielded receptors at >90%...

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Bibliographic Details
Main Authors: Baaske, Philipp (Author), Geissler, Sandra (Author), Wienken, Christoph J. (Author), Jerabek-Willemsen, Moran (Author), Duhr, Stefan (Author), Braun, Dieter (Author), Corin, Karolina A. (Contributor), Ravel, Deepali B. (Contributor), Song, Junyao (Contributor), Brown, Emily E. (Contributor), Wang, Xiaoqiang (Contributor), Zhang, Shuguang (Contributor)
Other Authors: Massachusetts Institute of Technology. Center for Biomedical Engineering (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor)
Format: Article
Language:English
Published: Public Library of Science, 2012-02-10T17:28:07Z.
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