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69658 |
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|a Grover, William H.
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|a Harvard University-
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|a Massachusetts Institute of Technology. Department of Biological Engineering
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|a Massachusetts Institute of Technology. Department of Materials Science and Engineering
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|a Massachusetts Institute of Technology. Department of Mechanical Engineering
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|a Koch Institute for Integrative Cancer Research at MIT
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|a Manalis, Scott R.
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|a Manalis, Scott R.
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|a Grover, William H.
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|a Bryan, Andrea Kristine
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|a Diez Silva, Monica
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|a Suresh, Subra
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|a Bryan, Andrea Kristine
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|a Diez Silva, Monica
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|a Suresh, Subra
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|a Higgins, John M.
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|a Manalis, Scott R.
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|a Measuring single-cell density
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|b National Academy of Sciences (U.S.),
|c 2012-03-14T20:43:08Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/69658
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|a We have used a microfluidic mass sensor to measure the density of single living cells. By weighing each cell in two fluids of different densities, our technique measures the single-cell mass, volume, and density of approximately 500 cells per hour with a density precision of 0.001 g mL-1. We observe that the intrinsic cell-to-cell variation in density is nearly 100-fold smaller than the mass or volume variation. As a result, we can measure changes in cell density indicative of cellular processes that would be otherwise undetectable by mass or volume measurements. Here, we demonstrate this with four examples: identifying Plasmodium falciparum malaria-infected erythrocytes in a culture, distinguishing transfused blood cells from a patient's own blood, identifying irreversibly sickled cells in a sickle cell patient, and identifying leukemia cells in the early stages of responding to a drug treatment. These demonstrations suggest that the ability to measure single-cell density will provide valuable insights into cell state for a wide range of biological processes.
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|a EUREKA (Exceptional, Unconventional Research Enabling Knowledge Acceleration (R01GM085457))
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|a National Institutes of Health (U.S.) (NIH Cell Decision Process Center Grant (P50GM68762))
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|a United States. Army Research Office (Institute for Collaborative Biotechnologies Grant (W911NF-09-D-0001))
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|a Massachusetts Institute of Technology (Whitaker Health Sciences Graduate Fellowship)
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|a National Institutes of Health (U.S.) (Grant R01HL094270)
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|a National Institute of Diabetes and Digestive and Kidney Diseases (U.S.) (Grant DK083242)
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|a en_US
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|a Article
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|t Proceedings of the National Academy of Sciences of the United States of America
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