Three-dimensional differential interference contrast microscopy using synthetic aperture imaging

We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image f...

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Bibliographic Details
Main Authors: Dasari, Ramachandra Rao (Contributor), Feld, Michael S. (Contributor), Sung, Yongjin (Contributor), Kim, Moonseok (Author), Choi, Youngwoon (Author), Fang-Yen, Chris (Author), Kim, Kwanhyung (Author), Choi, Wonshik (Author)
Other Authors: Massachusetts Institute of Technology. Spectroscopy Laboratory (Contributor)
Format: Article
Language:English
Published: SPIE, 2012-06-28T12:41:35Z.
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Online Access:Get fulltext
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042 |a dc 
100 1 0 |a Dasari, Ramachandra Rao  |e author 
100 1 0 |a Massachusetts Institute of Technology. Spectroscopy Laboratory  |e contributor 
100 1 0 |a Feld, Michael S.  |e contributor 
100 1 0 |a Dasari, Ramachandra Rao  |e contributor 
100 1 0 |a Feld, Michael S.  |e contributor 
100 1 0 |a Sung, Yongjin  |e contributor 
700 1 0 |a Feld, Michael S.  |e author 
700 1 0 |a Sung, Yongjin  |e author 
700 1 0 |a Kim, Moonseok  |e author 
700 1 0 |a Choi, Youngwoon  |e author 
700 1 0 |a Fang-Yen, Chris  |e author 
700 1 0 |a Kim, Kwanhyung  |e author 
700 1 0 |a Choi, Wonshik  |e author 
245 0 0 |a Three-dimensional differential interference contrast microscopy using synthetic aperture imaging 
260 |b SPIE,   |c 2012-06-28T12:41:35Z. 
856 |z Get fulltext  |u http://hdl.handle.net/1721.1/71243 
520 |a We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles. 
546 |a en_US 
655 7 |a Article 
773 |t Journal of Biomedical Optics