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|a Bajaj, Vikram S.
|e author
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|a Francis Bitter Magnet Laboratory
|q (Massachusetts Institute of Technology)
|e contributor
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|a Griffin, Robert Guy
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|a Bajaj, Vikram S.
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|a Mak-Jurkauskas, Melody L.
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|a Griffin, Robert Guy
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|a Mak-Jurkauskas, Melody L.
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|a Belenky, Marina
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|a Herzfeld, Judith
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|a Griffin, Robert Guy
|e author
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|a DNP Enhanced Frequency-Selective TEDOR Experiments in Bacteriorhodopsin
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|b Elsevier,
|c 2012-08-02T20:15:13Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/71967
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|a We describe a new approach to multiple [superscript 13]C-[superscript 15]N distance measurements in uniformly labeled solids, frequency-selective (FS) TEDOR. The method shares features with FS-REDOR and ZF- and BASE-TEDOR, which also provide quantitative [superscript 15]N-[superscript 13]C spectral assignments and distance measurements in U-[[superscript 13]C,[superscript 15]N] samples. To demonstrate the validity of the FS-TEDOR sequence, we measured distances in [U-[superscript 13]C,15N]-asparagine which are in good agreement with other methods. In addition, we integrate high frequency dynamic nuclear polarization (DNP) into the experimental protocol and use FS-TEDOR to record a resolved correlation spectrum of the Arg-[superscript 13]Cγ-[superscript 15]Nε region in [U-[superscript 13]C,15N]-bacteriorhodopsin. We resolve six of the seven cross-peaks expected based on the primary sequence of this membrane protein.
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|a National Institute of Biomedical Imaging and Bioengineering (U.S.) (Grant Number EB-001960)
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|a National Institute of Biomedical Imaging and Bioengineering (U.S.) (Grant Number EB-002804)
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|a National Institute of Biomedical Imaging and Bioengineering (U.S.) (Grant Number EB-001035)
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|a National Institute of Biomedical Imaging and Bioengineering (U.S.) (Grant Number EB-002026)
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|a en_US
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|a Article
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|t Journal of Magnetic Resonance
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