|
|
|
|
LEADER |
02287 am a22003013u 4500 |
001 |
71967 |
042 |
|
|
|a dc
|
100 |
1 |
0 |
|a Bajaj, Vikram S.
|e author
|
100 |
1 |
0 |
|a Francis Bitter Magnet Laboratory
|q (Massachusetts Institute of Technology)
|e contributor
|
100 |
1 |
0 |
|a Griffin, Robert Guy
|e contributor
|
100 |
1 |
0 |
|a Bajaj, Vikram S.
|e contributor
|
100 |
1 |
0 |
|a Mak-Jurkauskas, Melody L.
|e contributor
|
100 |
1 |
0 |
|a Griffin, Robert Guy
|e contributor
|
700 |
1 |
0 |
|a Mak-Jurkauskas, Melody L.
|e author
|
700 |
1 |
0 |
|a Belenky, Marina
|e author
|
700 |
1 |
0 |
|a Herzfeld, Judith
|e author
|
700 |
1 |
0 |
|a Griffin, Robert Guy
|e author
|
245 |
0 |
0 |
|a DNP Enhanced Frequency-Selective TEDOR Experiments in Bacteriorhodopsin
|
260 |
|
|
|b Elsevier,
|c 2012-08-02T20:15:13Z.
|
856 |
|
|
|z Get fulltext
|u http://hdl.handle.net/1721.1/71967
|
520 |
|
|
|a We describe a new approach to multiple [superscript 13]C-[superscript 15]N distance measurements in uniformly labeled solids, frequency-selective (FS) TEDOR. The method shares features with FS-REDOR and ZF- and BASE-TEDOR, which also provide quantitative [superscript 15]N-[superscript 13]C spectral assignments and distance measurements in U-[[superscript 13]C,[superscript 15]N] samples. To demonstrate the validity of the FS-TEDOR sequence, we measured distances in [U-[superscript 13]C,15N]-asparagine which are in good agreement with other methods. In addition, we integrate high frequency dynamic nuclear polarization (DNP) into the experimental protocol and use FS-TEDOR to record a resolved correlation spectrum of the Arg-[superscript 13]Cγ-[superscript 15]Nε region in [U-[superscript 13]C,15N]-bacteriorhodopsin. We resolve six of the seven cross-peaks expected based on the primary sequence of this membrane protein.
|
520 |
|
|
|a National Institute of Biomedical Imaging and Bioengineering (U.S.) (Grant Number EB-001960)
|
520 |
|
|
|a National Institute of Biomedical Imaging and Bioengineering (U.S.) (Grant Number EB-002804)
|
520 |
|
|
|a National Institute of Biomedical Imaging and Bioengineering (U.S.) (Grant Number EB-001035)
|
520 |
|
|
|a National Institute of Biomedical Imaging and Bioengineering (U.S.) (Grant Number EB-002026)
|
546 |
|
|
|a en_US
|
655 |
7 |
|
|a Article
|
773 |
|
|
|t Journal of Magnetic Resonance
|