Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo

• Main Authors: Mower, Amanda F. (Contributor), Yu, Hongbo (Contributor), Majewska, Ania K. (Contributor), Okamoto, Ken-Ichi (Contributor), Hayashi, Yasunori (Contributor), Sur, Mriganka (Contributor), Kwok, Show Ming (Author)
• Other Authors: Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), Picower Institute for Learning and Memory (Contributor), RIKEN-MIT Center for Neural Circuit Genetics (Contributor), Kwok, Showming (Contributor)
• Format: Article
• Language: English
• Published: National Academy of Sciences of the United States of America, 2012-09-20T20:51:13Z.
• Subjects: Article
• Online Access: Get fulltext

Unbalanced visual input during development induces persistent alterations in the function and structure of visual cortical neurons. The molecular mechanisms that drive activity-dependent changes await direct visualization of underlying signals at individual synapses in vivo. By using a genetically engineered Förster resonance energy transfer (FRET) probe for the detection of CaMKII activity, and two-photon imaging of single synapses within identified functional domains, we have revealed unexpected and differential mechanisms in specific subsets of synapses in vivo. Brief monocular deprivation leads to activation of CaMKII in most synapses of layer 2/3 pyramidal cells within deprived eye domains, despite reduced visual drive, but not in nondeprived eye domains. Synapses that are eliminated in deprived eye domains have low basal CaMKII activity, implying a protective role for activated CaMKII against synapse elimination.
National Basic Research Program of China (973 Program) (2010CB327901)
Japan. Ministry of Education, Culture, Sports, Science and Technology. Foundation of Synapse and Neurocircuit Pathology. (Grant-in-Aid for Scientific Research on Innovative Area)
RIKEN Brain Science Institute
National Institutes of Health (U.S.) (Grant R01DA17310)
National Institutes of Health (U.S.) (Postdoctoral Fellowship)
Japan Society for the Promotion of Science (Grant-In-Aid for Scientific Research)