Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters

• Main Authors: Lukovic, Elvedin (Contributor), Taylor, Elizabeth Vogel (Contributor), Imperiali, Barbara (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Chemistry (Contributor)
• Format: Article
• Language: English
• Published: Wiley Blackwell (John Wiley & Sons), 2012-10-10T14:57:05Z.
• Subjects: Article
• Online Access: Get fulltext

2010 September 13
The best of both worlds: Combining a recombinant docking domain (see picture, blue ribbon) with a chemical sensing module (green hexagons) yields a highly selective ERK sensor. This probe exclusively monitors ERK1/2 activity in unfractionated cell lysates without any off-target kinase inhibitors. Good selectivity and biophysical parameters allow high-throughput analysis of ERK1/2 activity without time-consuming enzyme purification.
National Institutes of Health (U.S.). Cell Migration Consortium (GM064346)
National Science Foundation (U.S.). Biophysical Instrumentation Facility for the Study of Complex Macromolecular Systems (NSF-0070319)