Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters
2010 September 13
Main Authors: | , , |
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Other Authors: | , |
Format: | Article |
Language: | English |
Published: |
Wiley Blackwell (John Wiley & Sons),
2012-10-10T14:57:05Z.
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Subjects: | |
Online Access: | Get fulltext |
Summary: | 2010 September 13 The best of both worlds: Combining a recombinant docking domain (see picture, blue ribbon) with a chemical sensing module (green hexagons) yields a highly selective ERK sensor. This probe exclusively monitors ERK1/2 activity in unfractionated cell lysates without any off-target kinase inhibitors. Good selectivity and biophysical parameters allow high-throughput analysis of ERK1/2 activity without time-consuming enzyme purification. National Institutes of Health (U.S.). Cell Migration Consortium (GM064346) National Science Foundation (U.S.). Biophysical Instrumentation Facility for the Study of Complex Macromolecular Systems (NSF-0070319) |
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