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73713 |
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|a dc
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|a Lukovic, Elvedin
|e author
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|a Massachusetts Institute of Technology. Department of Biology
|e contributor
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|a Massachusetts Institute of Technology. Department of Chemistry
|e contributor
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|a Lukovic, Elvedin
|e contributor
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|a Taylor, Elizabeth Vogel
|e contributor
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|a Imperiali, Barbara
|e contributor
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|a Taylor, Elizabeth Vogel
|e author
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|a Imperiali, Barbara
|e author
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|a Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters
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|b Wiley Blackwell (John Wiley & Sons),
|c 2012-10-10T14:57:05Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/73713
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|a 2010 September 13
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|a The best of both worlds: Combining a recombinant docking domain (see picture, blue ribbon) with a chemical sensing module (green hexagons) yields a highly selective ERK sensor. This probe exclusively monitors ERK1/2 activity in unfractionated cell lysates without any off-target kinase inhibitors. Good selectivity and biophysical parameters allow high-throughput analysis of ERK1/2 activity without time-consuming enzyme purification.
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|a National Institutes of Health (U.S.). Cell Migration Consortium (GM064346)
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|a National Science Foundation (U.S.). Biophysical Instrumentation Facility for the Study of Complex Macromolecular Systems (NSF-0070319)
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|a en_US
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|a Article
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|t Angewandte Chemie International Edition
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