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|a Brohawn, Stephen G.
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|a Massachusetts Institute of Technology. Department of Biology
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|a Brohawn, Stephen G.
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|a Schwartz, Thomas
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|a Schwartz, Thomas
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|a Molecular architecture of the Nup84-Nup145C-Sec13 edge element in the nuclear pore complex lattice
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|b Nature Publishing Group,
|c 2012-11-01T20:20:39Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/74558
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|a Nuclear pore complexes (NPCs) facilitate all nucleocytoplasmic transport. These massive protein assemblies are modular, with a stable structural scaffold supporting more dynamically attached components. The scaffold is made from multiple copies of the heptameric Y complex and the heteromeric Nic96 complex. We previously showed that members of these core subcomplexes specifically share an ACE1 fold with Sec31 of the COPII vesicle coat, and we proposed a lattice model for the NPC based on this commonality. Here we present the crystal structure of the heterotrimeric 134-kDa complex of Nup84-Nup145C-Sec13 of the Y complex. The heterotypic ACE1 interaction of Nup84 and Nup145C is analogous to the homotypic ACE1 interaction of Sec31 that forms COPII lattice edge elements and is inconsistent with the alternative 'fence-like' NPC model. We construct a molecular model of the Y complex and compare the architectural principles of COPII and NPC lattices.
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|a National Institutes of Health (U.S.) (Grant GM77537)
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|a Pew Charitable Trusts (Scholar Award)
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|a en_US
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|a Article
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|t Nature Structural & Molecular Biology
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