Nanoparticulate Cellular Patches for Cell-Mediated Tumoritropic Delivery

• Main Authors: Cheng, Hao (Author), Kastrup, Christian (Author), Ramanathan, Renuka (Contributor), Siegwart, Daniel J. (Author), Ma, Minglin (Author), Bogatyrev, Said R. (Author), Whitehead, Kathryn A. (Author), Langer, Robert (Author), Anderson, Daniel G. (Author)
• Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor)
• Format: Article
• Language: English
• Published: American Chemical Society (ACS), 2012-12-07T20:51:59Z.
• Subjects: Article
• Online Access: Get fulltext

Author Manuscript: 2011 February 23.
The targeted delivery of therapeutics to tumors remains an important challenge in cancer nanomedicine. Attaching nanoparticles to cells that have tumoritropic migratory properties is a promising modality to address this challenge. Here we describe a technique to create nanoparticulate cellular patches that remain attached to the membrane of cells for up to 2 days. NeutrAvidin-coated nanoparticles were anchored on cells possessing biotinylated plasma membrane. Human bone marrow derived mesenchymal stem cells with nanoparticulate patches retained their inherent tumoritropic properties as shown using a tumor model in a 3D extracellular matrix. Additionally, human umbilical vein endothelial cells with nanoparticulate patches were able to retain their functional properties and form multicellular structures as rapidly as unmodified endothelial cells. These results provide a novel strategy to actively deliver nanostructures and therapeutics to tumors utilizing stem cells as carriers and also suggest that nanoparticulate cellular patches may have applications in tissue regeneration.
United States. Defense Advanced Research Projects Agency (Grant W911NF-07-1-0210)
National Institutes of Health (U.S.) (Grant EB000244)
United States. Defense Advanced Research Projects Agency (Grant 2-P30- CA14051)