Efficient replication bypass of size-expanded DNA base pairs in bacterial cells

• Main Authors: Delaney, James C. (Contributor), Gao, Jianmin (Author), Liu, Haibo (Author), Shrivastav, Nidhi (Contributor), Essigmann, John M. (Contributor), Kool, Eric T. (Author)
• Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Chemistry (Contributor)
• Format: Article
• Language: English
• Published: Wiley Blackwell, 2012-12-12T18:48:18Z.
• Subjects: Article
• Online Access: Get fulltext

Author Manuscript 2012 September 06
Supersize me! Size-expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners.
National Institutes of Health (U.S.) (CA80024)
National Institutes of Health (U.S.) (GM63587)
Stanford Graduate Fellowship